Design of pH sensitive binding proteins from the hyperthermophilic Sso7d scaffold.

Design of pH sensitive binding proteins from the hyperthermophilic Sso7d scaffold.

We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG) (hFc) using two different strategies - histidine scanning and random mutagenesis. We obtained an hFc-binding protein, Sso7d-hFc, through mutagenesis of the Sso7d protein from the hyperthermophilic ar...

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Bibliographic Details
Main Authors: Nimish Gera, Andrew B Hill, Dalon P White, Ruben G Carbonell, Balaji M Rao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3492137?pdf=render

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