Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria

Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria

Transcription by bacterial RNA polymerase is interrupted by pausing events that play diverse regulatory roles. Here, the authors find that a large number of E. coli sigma70-dependent pauses, clustered at a 10−20-bp distance from promoters, are regulated by Gre cleavage factors constituting a mechani...

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Main Authors: Zhe Sun, Alexander V. Yakhnin, Peter C. FitzGerald, Carl E. Mclntosh, Mikhail Kashlev
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21150-2
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spelling doaj-a3fc4f3397f444a2a4e04ebfd6d6f34c2021-02-14T12:12:24ZengNature Publishing GroupNature Communications2041-17232021-02-0112111410.1038/s41467-021-21150-2Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteriaZhe Sun0Alexander V. Yakhnin1Peter C. FitzGerald2Carl E. Mclntosh3Mikhail Kashlev4RNA Biology Laboratory, National Cancer Institute, National Institutes of HealthRNA Biology Laboratory, National Cancer Institute, National Institutes of HealthGenome Analysis Unit, National Cancer Institute, National Institutes of HealthGenome Analysis Unit, National Cancer Institute, National Institutes of HealthRNA Biology Laboratory, National Cancer Institute, National Institutes of HealthTranscription by bacterial RNA polymerase is interrupted by pausing events that play diverse regulatory roles. Here, the authors find that a large number of E. coli sigma70-dependent pauses, clustered at a 10−20-bp distance from promoters, are regulated by Gre cleavage factors constituting a mechanism for rapid response to changing environmental cues.https://doi.org/10.1038/s41467-021-21150-2
collection DOAJ
language English
format Article
sources DOAJ
author Zhe Sun
Alexander V. Yakhnin
Peter C. FitzGerald
Carl E. Mclntosh
Mikhail Kashlev
spellingShingle Zhe Sun
Alexander V. Yakhnin
Peter C. FitzGerald
Carl E. Mclntosh
Mikhail Kashlev
Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria
Nature Communications
author_facet Zhe Sun
Alexander V. Yakhnin
Peter C. FitzGerald
Carl E. Mclntosh
Mikhail Kashlev
author_sort Zhe Sun
title Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria
title_short Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria
title_full Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria
title_fullStr Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria
title_full_unstemmed Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria
title_sort nascent rna sequencing identifies a widespread sigma70-dependent pausing regulated by gre factors in bacteria
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-02-01
description Transcription by bacterial RNA polymerase is interrupted by pausing events that play diverse regulatory roles. Here, the authors find that a large number of E. coli sigma70-dependent pauses, clustered at a 10−20-bp distance from promoters, are regulated by Gre cleavage factors constituting a mechanism for rapid response to changing environmental cues.
url https://doi.org/10.1038/s41467-021-21150-2
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AT alexandervyakhnin nascentrnasequencingidentifiesawidespreadsigma70dependentpausingregulatedbygrefactorsinbacteria
AT petercfitzgerald nascentrnasequencingidentifiesawidespreadsigma70dependentpausingregulatedbygrefactorsinbacteria
AT carlemclntosh nascentrnasequencingidentifiesawidespreadsigma70dependentpausingregulatedbygrefactorsinbacteria
AT mikhailkashlev nascentrnasequencingidentifiesawidespreadsigma70dependentpausingregulatedbygrefactorsinbacteria
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