A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum

A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum

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This work developed a novel nested polymerase chain reaction (n-PCR) assay to identify Sorghum nitidum (S. nitidum). It has been designed a set of specific n-PCR inner primers Snit5/Snit2 and outer primers Nout1/Nout2 based on a conserved nucleotide sequence of adh1-like gene of S. nitidum. Fourteen...

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Main Authors: Shasha WEI, Zhirui DENG, Liping YIN, Jianping YI, Renqi WU, Qin CHEN
Format: Article
Language:English
Published: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 2011-11-01
Series:Notulae Scientia Biologicae
Online Access:http://www.notulaebiologicae.ro/index.php/nsb/article/view/6329
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spelling doaj-a3b8afe2ee304c81ac55d415731210082020-11-25T01:08:25ZengUniversity of Agricultural Sciences and Veterinary Medicine, Cluj-NapocaNotulae Scientia Biologicae2067-32052067-32642011-11-013414314610.15835/nsb3463296785A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidumShasha WEI0Zhirui DENG1Liping YIN2Jianping YI3Renqi WU4Qin CHEN5Shanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, ShanghaiShanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, ShanghaiShanghai Entry-Exit Quarantine and Inspection Bureau, 200135, ShanghaiShanghai Entry-Exit Quarantine and Inspection Bureau, 200135, ShanghaiShanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, ShanghaiShanghai University, School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, 200444, ShanghaiThis work developed a novel nested polymerase chain reaction (n-PCR) assay to identify Sorghum nitidum (S. nitidum). It has been designed a set of specific n-PCR inner primers Snit5/Snit2 and outer primers Nout1/Nout2 based on a conserved nucleotide sequence of adh1-like gene of S. nitidum. Fourteen samples of sorghum were used to investigate the specificity of the primers and the n-PCR assay. The result showed that 9 samples of S. nitidum displayed a positive strong, specific amplified band at ~873 bp in gel spectra, while other relatives, including Sorghum halepense, Sorghum almum, Sorghum bicolor, Sorghum propinum and Sorghum sudanse exhibited negative amplifications. This assay was able to specifically identify S. nitidum fast and effectively, which could be applied widely in field inspection, agriculture production and plant protection.http://www.notulaebiologicae.ro/index.php/nsb/article/view/6329
collection DOAJ
language English
format Article
sources DOAJ
author Shasha WEI
Zhirui DENG
Liping YIN
Jianping YI
Renqi WU
Qin CHEN
spellingShingle Shasha WEI
Zhirui DENG
Liping YIN
Jianping YI
Renqi WU
Qin CHEN
A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum
Notulae Scientia Biologicae
author_facet Shasha WEI
Zhirui DENG
Liping YIN
Jianping YI
Renqi WU
Qin CHEN
author_sort Shasha WEI
title A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum
title_short A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum
title_full A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum
title_fullStr A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum
title_full_unstemmed A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum
title_sort novel nested polymerase chain reaction (n-pcr) assay for identifying sorghum nitidum
publisher University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
series Notulae Scientia Biologicae
issn 2067-3205
2067-3264
publishDate 2011-11-01
description This work developed a novel nested polymerase chain reaction (n-PCR) assay to identify Sorghum nitidum (S. nitidum). It has been designed a set of specific n-PCR inner primers Snit5/Snit2 and outer primers Nout1/Nout2 based on a conserved nucleotide sequence of adh1-like gene of S. nitidum. Fourteen samples of sorghum were used to investigate the specificity of the primers and the n-PCR assay. The result showed that 9 samples of S. nitidum displayed a positive strong, specific amplified band at ~873 bp in gel spectra, while other relatives, including Sorghum halepense, Sorghum almum, Sorghum bicolor, Sorghum propinum and Sorghum sudanse exhibited negative amplifications. This assay was able to specifically identify S. nitidum fast and effectively, which could be applied widely in field inspection, agriculture production and plant protection.
url http://www.notulaebiologicae.ro/index.php/nsb/article/view/6329
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