Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals

Background: In this study, the first attempt in Basrah city to isolate and diagnose H. pylori from endoscopic units and workers by culture method. Objective: to isolate and identify H. pylori by culture method and conventional tests from possibly contaminated sources and workers in endoscopy units...

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Main Authors: Amani A. AL-Abdula, Saad S. AL- Tahir and Amin A. AL-Sulami
Format: Article
Language:English
Published: University of Basrah 2013-12-01
Series:The Medical Journal of Basrah University
Subjects:
Online Access:https://mjbu.uobasrah.edu.iq/article_90714_82aa185d2151dd4a7c6af3b3c817d682.pdf
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spelling doaj-a371aee901ad49a3895539642026ee742020-11-25T03:13:26ZengUniversity of BasrahThe Medical Journal of Basrah University 0253-07592413-44142013-12-01312616710.33762/mjbu.2013.9071490714Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra HospitalsAmani A. AL-AbdulaSaad S. AL- Tahir and Amin A. AL-SulamiBackground: In this study, the first attempt in Basrah city to isolate and diagnose H. pylori from endoscopic units and workers by culture method. Objective: to isolate and identify H. pylori by culture method and conventional tests from possibly contaminated sources and workers in endoscopy units in five hospitals. Materials and methods: A total of 78 swabs were collected endoscopic units in five hospitals in Basrah city in Iraq namely: Altaalemi, Al-mawani, Al-Basrah general, Al-Shifaa and Al-Fayhaa during the period from September 2011 to June 2012. Results: A total of 78 swabs, of which 12(15.38%) isolates of H. pylori were isolated by culture method and identified by biochemical tests. The highest frequency of H. pylori was in Altaalemi 7(29.16%) followed by Al-Shifaa 3(25%) and then Al-mawani 2(14.2%) and zero for the rest. The frequency of positive H. pylori in 13 samples from mouth of staff, was 3(23.1%). It was statistically significant (P<0.05) using SPSS program. Of the total 10 samples from forceps, 2(20%) showed positive tests for H. pylori, the frequency of positive H. pylori in 3 samples from sponge, was 2(66.7%), of the total 8 samples from flexible shaft, 2(25%) showed positive tests for H. pylori, 6 specimens from flexible cord transmitting light, air and water, 1(16.7%) showed positive tests for H. pylori, 5 specimens from Petridis for mouth pieces and 5 specimens from opening for biopsy channel, 1(20%) showed positive tests for H. pylori, Of the total 10, 6, 5, 5 and 2 samples from mouth, tables surface, beds, sinks and Pail sterilization respectively, the frequency of H. pylori was (0%). Conclusion: H. pylori can be isolated by culture method from endoscopic units and workers and disinfection technique for manual cleaning and disinfection of the instrument may not remove the risk of H. pylori transmission.https://mjbu.uobasrah.edu.iq/article_90714_82aa185d2151dd4a7c6af3b3c817d682.pdfhelicobacter pyloriisolatediagnoseculture method
collection DOAJ
language English
format Article
sources DOAJ
author Amani A. AL-Abdula
Saad S. AL- Tahir and Amin A. AL-Sulami
spellingShingle Amani A. AL-Abdula
Saad S. AL- Tahir and Amin A. AL-Sulami
Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals
The Medical Journal of Basrah University
helicobacter pylori
isolate
diagnose
culture method
author_facet Amani A. AL-Abdula
Saad S. AL- Tahir and Amin A. AL-Sulami
author_sort Amani A. AL-Abdula
title Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals
title_short Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals
title_full Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals
title_fullStr Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals
title_full_unstemmed Culture method for isolation of Helicobacter pylori contamination of Endoscopic Units in Basra Hospitals
title_sort culture method for isolation of helicobacter pylori contamination of endoscopic units in basra hospitals
publisher University of Basrah
series The Medical Journal of Basrah University
issn 0253-0759
2413-4414
publishDate 2013-12-01
description Background: In this study, the first attempt in Basrah city to isolate and diagnose H. pylori from endoscopic units and workers by culture method. Objective: to isolate and identify H. pylori by culture method and conventional tests from possibly contaminated sources and workers in endoscopy units in five hospitals. Materials and methods: A total of 78 swabs were collected endoscopic units in five hospitals in Basrah city in Iraq namely: Altaalemi, Al-mawani, Al-Basrah general, Al-Shifaa and Al-Fayhaa during the period from September 2011 to June 2012. Results: A total of 78 swabs, of which 12(15.38%) isolates of H. pylori were isolated by culture method and identified by biochemical tests. The highest frequency of H. pylori was in Altaalemi 7(29.16%) followed by Al-Shifaa 3(25%) and then Al-mawani 2(14.2%) and zero for the rest. The frequency of positive H. pylori in 13 samples from mouth of staff, was 3(23.1%). It was statistically significant (P<0.05) using SPSS program. Of the total 10 samples from forceps, 2(20%) showed positive tests for H. pylori, the frequency of positive H. pylori in 3 samples from sponge, was 2(66.7%), of the total 8 samples from flexible shaft, 2(25%) showed positive tests for H. pylori, 6 specimens from flexible cord transmitting light, air and water, 1(16.7%) showed positive tests for H. pylori, 5 specimens from Petridis for mouth pieces and 5 specimens from opening for biopsy channel, 1(20%) showed positive tests for H. pylori, Of the total 10, 6, 5, 5 and 2 samples from mouth, tables surface, beds, sinks and Pail sterilization respectively, the frequency of H. pylori was (0%). Conclusion: H. pylori can be isolated by culture method from endoscopic units and workers and disinfection technique for manual cleaning and disinfection of the instrument may not remove the risk of H. pylori transmission.
topic helicobacter pylori
isolate
diagnose
culture method
url https://mjbu.uobasrah.edu.iq/article_90714_82aa185d2151dd4a7c6af3b3c817d682.pdf
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