Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test

• Main Authors: Mohamed Hamidouche, Nassira Belmessabih, Abderrahmane Boubguira, Amina Benfenatki, Naim Saada, Amina Sail, Fatima Bacha
• Format: Article
• Language: English
• Published: Tripoli University 2018-09-01
• Series: Open Veterinary Journal
• Subjects: Capripoxvirus; Embryonic lamb kidney cells; RM65 strain; Vaccine effectiveness; Virus neutralisation
• Online Access: https://www.openveterinaryjournal.com/OVJ-2017-10-198%20M.%20Hamidouche%20et%20al.pdf

Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (TAb) respectively. The authors have demonstrated that a positive correlation exists between N.I. and TAb values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log TAb) + 1.331; this serves as a base to calculate N.I. using TAb values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively.