The effect of bioresorbable additives and micro-bioobjects on gel formation, stabilization and thermophysical properties

• Main Authors: Pokusaev Boris, Vyazmin Andrey, Zakharov Nikolay, Karlov Sergey, Nekrasov Dmitry, Reznik Vyacheslav, Khramtsov Dmitry
• Format: Article
• Language: English
• Published: VINCA Institute of Nuclear Sciences 2019-01-01
• Series: Thermal Science
• Subjects: bioprinting; agarose gel; bioresorbable additive; cells; spectroscopy; kinetics of formation; gel aging; evaporation; thermophysical properties of gel
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-9836/2019/0354-98361800350P.pdf

The same properties of agarose gels containing neutral bioresorbable additives and living microorganisms which are important for use in additive technologies of bioreactors creation were considered. Data on the kinetics of gel formation from the solution during cooling were obtained by spectroscopic measurement by measuring the shift of the maximum spectrum of light passing through the gel, depending on the temperature. The dynamics of aging was investigated for gels of different concentrations of agarose, bioresorbable additives and living cells. The time dependences of the decrease in the optical transparency of such gels during the aging process, characterizing the changes in their structure, were obtained. Special attention was paid to the effect of liquid evaporation from gels in the process of gel formation and during long-term storage on relaxation processes leading to their spontaneous increase in density. Experiments were performed to determine the dynamics of the temperature fields simultaneously with heat flux measurements during the formation of studied gels with different concentrations. On the basis of the obtained experimental data and previously developed method, the thermophysical coefficients of agarose gels containing an admixture of starch and living yeast cells were calculated.