Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region
Harmful cyanobacterial blooms have become increasingly common in freshwater ecosystems in recent decades, mainly due to eutrophication and climate change. Water becomes unreliable for human consumption. Here, we report a comprehensive study carried out to investigate the water quality of several Cam...
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doaj-a34e1e52aae848ed97ede274587e9fbf2020-11-24T22:15:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00176318788Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid RegionJuline M. Walter0Fabyano A. C. Lopes1Mônica Lopes-Ferreira2Lívia M. Vidal3Luciana Leomil4Fabiana Melo5Girlene S. de Azevedo6Rossandra M. S. Oliveira7Alba J. Medeiros8Adriana S. O. Melo9Carlos E. De Rezende10Amilcar Tanuri11Fabiano L. Thompson12Laboratory of Microbiology, Institute of Biology, and SAGE-COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Microbiology, Institute of Biology, and SAGE-COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilImmunoregulation Unit, Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, BrazilLaboratory of Microbiology, Institute of Biology, and SAGE-COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Microbiology, Institute of Biology, and SAGE-COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Pesquisa Professor Joaquim Amorim Neto, Campina Grande, BrazilInstituto de Pesquisa Professor Joaquim Amorim Neto, Campina Grande, BrazilSecretaria de Saúde de Campina Grande, Campina Grande, BrazilSecretaria de Saúde de Campina Grande, Campina Grande, BrazilInstituto de Pesquisa Professor Joaquim Amorim Neto, Campina Grande, BrazilLaboratory of Environmental Sciences, State University of Northern Rio de Janeiro, Campos dos Goytacazes, BrazilLaboratory of Virology, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Microbiology, Institute of Biology, and SAGE-COPPE, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilHarmful cyanobacterial blooms have become increasingly common in freshwater ecosystems in recent decades, mainly due to eutrophication and climate change. Water becomes unreliable for human consumption. Here, we report a comprehensive study carried out to investigate the water quality of several Campina Grande reservoirs. Our approach included metagenomics, microbial abundance quantification, ELISA test for three cyanotoxins (microcystin, nodularins, and cylindrospermopsin), and in vivo ecotoxicological tests with zebrafish embryos. Cytometry analysis showed high cyanobacterial abundance, while metagenomics identified an average of 10.6% of cyanobacterial sequences, and demonstrated the presence of Microcystis, Cylindrospermopsis, and toxin coding genes in all ponds. Zebrafish embryos reared with pond water had high mortality and diverse malformations. Among the ponds analyzed, Araçagi showed the highest lethality (an average of 62.9 ± 0.8%), followed by Boqueirão (lethality average of 62.5 ± 0.8%). Here, we demonstrate that water from ponds undergoing extremely drought conditions have an abundance of potentially harmful cyanobacteria and their toxins. Our findings are consistent with a scenario in which polluted drinking water poses a great risk to human health.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00176/fullcyanotoxinszebrafishmetagenomicspublic healthyeutrophication |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juline M. Walter Fabyano A. C. Lopes Mônica Lopes-Ferreira Lívia M. Vidal Luciana Leomil Fabiana Melo Girlene S. de Azevedo Rossandra M. S. Oliveira Alba J. Medeiros Adriana S. O. Melo Carlos E. De Rezende Amilcar Tanuri Fabiano L. Thompson |
spellingShingle |
Juline M. Walter Fabyano A. C. Lopes Mônica Lopes-Ferreira Lívia M. Vidal Luciana Leomil Fabiana Melo Girlene S. de Azevedo Rossandra M. S. Oliveira Alba J. Medeiros Adriana S. O. Melo Carlos E. De Rezende Amilcar Tanuri Fabiano L. Thompson Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region Frontiers in Microbiology cyanotoxins zebrafish metagenomics public healthy eutrophication |
author_facet |
Juline M. Walter Fabyano A. C. Lopes Mônica Lopes-Ferreira Lívia M. Vidal Luciana Leomil Fabiana Melo Girlene S. de Azevedo Rossandra M. S. Oliveira Alba J. Medeiros Adriana S. O. Melo Carlos E. De Rezende Amilcar Tanuri Fabiano L. Thompson |
author_sort |
Juline M. Walter |
title |
Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region |
title_short |
Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region |
title_full |
Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region |
title_fullStr |
Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region |
title_full_unstemmed |
Occurrence of Harmful Cyanobacteria in Drinking Water from a Severely Drought-Impacted Semi-arid Region |
title_sort |
occurrence of harmful cyanobacteria in drinking water from a severely drought-impacted semi-arid region |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-02-01 |
description |
Harmful cyanobacterial blooms have become increasingly common in freshwater ecosystems in recent decades, mainly due to eutrophication and climate change. Water becomes unreliable for human consumption. Here, we report a comprehensive study carried out to investigate the water quality of several Campina Grande reservoirs. Our approach included metagenomics, microbial abundance quantification, ELISA test for three cyanotoxins (microcystin, nodularins, and cylindrospermopsin), and in vivo ecotoxicological tests with zebrafish embryos. Cytometry analysis showed high cyanobacterial abundance, while metagenomics identified an average of 10.6% of cyanobacterial sequences, and demonstrated the presence of Microcystis, Cylindrospermopsis, and toxin coding genes in all ponds. Zebrafish embryos reared with pond water had high mortality and diverse malformations. Among the ponds analyzed, Araçagi showed the highest lethality (an average of 62.9 ± 0.8%), followed by Boqueirão (lethality average of 62.5 ± 0.8%). Here, we demonstrate that water from ponds undergoing extremely drought conditions have an abundance of potentially harmful cyanobacteria and their toxins. Our findings are consistent with a scenario in which polluted drinking water poses a great risk to human health. |
topic |
cyanotoxins zebrafish metagenomics public healthy eutrophication |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00176/full |
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