NANOSTRUCTURED FERRIC CITRATE EFFECT ON Chlorella vulgaris DEVELOPMENT

• Main Authors: N. B. Golub, M. Tsvetkovych, I. I. Levtun, V. I. Maksyn
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2018-12-01
• Series: Biotechnologia Acta
• Subjects: chlorella cells; biomass; intracellular iron; chlorophylls; carotenoids; nanostructured ferric citrate.
• Online Access: http://biotechnology.kiev.ua/images/storage/6_2018/golub_6_2018.pdf

The aim of the research was to study the development of Chlorella vulgaris at culturing on the modified Gromov 6 medium with high concentrations of nanostructured ferric citrate and also its effect on photosynthesis pigments accumulation. It was demonstrated that the highest intracellular iron content (15 mg/g of dry mass) in the culture cells was typical with nanostructured ferric citrate content of 30 mg/dm3 of culture medium, the highest content of chlorophyll a — 23 mg/g of dry algae mass, b — 7.5 mg/g of dry mass, and carotenoids — 9.2 mg/g of dry mass was observed at nanostructured ferric citrate content of 20 mg/dm3. The use of nanostructured ferric citrate leaded to an increase in the chlorella biomass yield by 3 times with compared to standard technology. Simultaneously, intracellular iron content in cells increased significantly with the use of nanostructured ferric citrate, which increases their value as a nutritional supplement. In order to increase the biomass yield and intracellular iron content in cells, application of nanostructured ferric citrate is recommended.