Circulating microRNA changes in patients with impaired glucose regulation

• Main Authors: Helene A. Fachim, Camila M. Loureiro, Kirk Siddals, Caroline F Dalton, Gavin P. Reynolds, J. Martin Gibson, Zhen Bouman Chen, Adrian H. Heald
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2020-01-01
• Series: Adipocyte
• Subjects: circulating micrornas; pre-diabetes; impaired glucose regulation (igr); lifestyle change
• Online Access: http://dx.doi.org/10.1080/21623945.2020.1798632

We analysed if levels of four miRNAs would change after a lifestyle intervention involving dietary and exercises in prediabetes. MiRNAs previously shown to be associated with diabetes (Let-7a, Let-7e, miR-144 and miR-92a) were extracted from serum pre- and post-intervention. mRNA was extracted from fat-tissue for gene expression analyses. The intervention resulted in increased Let-7a and miR-92a. We found correlations between miRNAs and clinical variables (triglycerides, cholesterol, insulin, weight and BMI). We also found correlations between miRNAs and target genes, revealing a link between miR-92a and IGF system. A lifestyle intervention resulted in marked changes in miRNAs. The association of miRNAs with insulin and the IGF system (both receptors and binding proteins) may represent a mechanism of regulating IGFs metabolic actions.