Cranberry extract suppresses interleukin-8 secretion from stomach cells stimulated by Helicobacter pylori in every clinically separated strain but inhibits growth in part of the strains

• Main Authors: Masashi Matsushima, Takayoshi Suzuki, Aya Masui, Tetsuya Mine, Atsushi Takagi
• Format: Article
• Language: English
• Published: Elsevier 2013-04-01
• Series: Journal of Functional Foods
• Subjects: Helicobacter pylori; Cranberry; Functional food products; Growth; IL-8; Strain
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464613000364
• ISSN: 1756-4646

It is known that cranberry inhibits the growth of Helicobacter pylori (HP). In human stomach, HP basically induces chronic inflammation by stimulating stomach cells to secrete interleukin (IL)-8 and other inflammatory cytokines, and causes stomach cancer, etc. The aim of this study was to investigate the inhibiting effects of cranberry on HP growth and IL-8 secretion from stomach cells induced by HP, using clinically separated HP strains. HP growth in liquid culture and on-plate culture was evaluated by titration after 2-day incubation and by agar dilution technique, respectively. For IL-8 experiments, MKN-45, a stomach cancer cell line, was incubated with HP for 24 h and IL-8 in the medium was assayed by ELISA. Cranberry suppressed growth of the bacteria only in six of the 27 strains. Meanwhile, it suppressed IL-8 secretion in all the strains. The results may suggest a possible role of cranberry in prevention of stomach cancer by reducing gastric inflammation.