Tetra-primer ARMS-PCR identified a missense mutation of the bovine <i>NRIP1</i> gene associated with growth traits

• Main Authors: D. Liu, Z. Wang, W. Ma, Y. Gao, A. Li, X. Lan, C. Lei, H. Chen
• Format: Article
• Language: English
• Published: Copernicus Publications 2015-04-01
• Series: Archives Animal Breeding
• Online Access: http://www.arch-anim-breed.net/58/165/2015/aab-58-165-2015.pdf

Nuclear receptor-interacting protein 1 (<i>NRIP1</i>) specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors to inhibit transcription. Previous work has demonstrated this protein to be a key regulator in modulating transcriptional activity of many transcription factors, some of which are closely related to development and growth. In this study, we have successfully genotyped two newly identified bovine <i>NRIP1</i> single-nucleotide polymorphisms (SNPs) (c.605A > G and c.1301G > A) using the T-ARMS-PCR method and validated the accuracy by means of PCR-RFLP assay using 1809 individuals of 9 different cattle breeds. The association analyses results indicated that c.605A > G locus was significantly associated with body weight and average daily gain in Nanyang cattle at 18 months (<i>P</i> < 0.05). Thus it can be inferred that T-ARMS-PCR is a rapid, reliable, and cheap method for SNP genotyping and that c.605A > G polymorphism in bovine <i>NRIP1</i> is associated with growth traits. These findings will be of benefit for the application of DNA markers related to growth traits in marker-assisted selection (MAS), and will improve the promotion of beef cattle.