Development and validation of a method for the analysis of tetracyclines in chicken-muscle by liquid chromatography-electrospray-mass spectrometry in tandem (LC-ESI-MS/MS)

• Main Authors: Thais Matsue Uekane, Francisco Radler Aquino Neto, Luiz Nelson F Gomes
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2011-01-01
• Series: Química Nova
• Subjects: tetracyclines; chicken-muscle; validation
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000100009&lng=en&tlng=en

A LC-ESI-MS/MS method was developed and validated according to the European Union decision 2002/657/EC, for the determination of tetracyclines (TCs) in chicken-muscle since Europe is one of the main markets for Brazilian products. Linearity of r > 0.9979, limits of quantification in the range of 7.0-35.0 ng/g, average recoveries of 89.38 - 106.27%, within-day and between-day precision were adequate for all TCs. The decision limit and the detection capability were 93.00-106.46 ng/g and 95.84-114.38 ng/g, respectively. This method is suitable for application in surveillance programmes of residues of TCs in chicken-muscle samples.