A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions

A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions

Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness...

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Main Authors: Ulrich Schlecht, Zhimin Liu, Jamie R. Blundell, Robert P. St.Onge, Sasha F. Levy
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15586
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spelling doaj-a2d1f7e3c1634cb29a0d1f9355ce7b112021-05-11T07:30:37ZengNature Publishing GroupNature Communications2041-17232017-05-01811910.1038/ncomms15586A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactionsUlrich Schlecht0Zhimin Liu1Jamie R. Blundell2Robert P. St.Onge3Sasha F. Levy4Stanford Genome Technology Center, Stanford UniversityLaufer Center for Physical and Quantitative Biology, Stony Brook UniversityLaufer Center for Physical and Quantitative Biology, Stony Brook UniversityStanford Genome Technology Center, Stanford UniversityLaufer Center for Physical and Quantitative Biology, Stony Brook UniversityProtein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness from barcode lineage trajectories.https://doi.org/10.1038/ncomms15586
collection DOAJ
language English
format Article
sources DOAJ
author Ulrich Schlecht
Zhimin Liu
Jamie R. Blundell
Robert P. St.Onge
Sasha F. Levy
spellingShingle Ulrich Schlecht
Zhimin Liu
Jamie R. Blundell
Robert P. St.Onge
Sasha F. Levy
A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
Nature Communications
author_facet Ulrich Schlecht
Zhimin Liu
Jamie R. Blundell
Robert P. St.Onge
Sasha F. Levy
author_sort Ulrich Schlecht
title A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_short A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_full A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_fullStr A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_full_unstemmed A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
title_sort scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-05-01
description Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness from barcode lineage trajectories.
url https://doi.org/10.1038/ncomms15586
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