Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products

Among others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU proj...

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Main Authors:	Monica Caffara, Andrea Gustinelli, Angelica Mazzone, Maria L. Fioravanti
Format:	Article
Language:	English
Published:	Elsevier 2020-06-01
Series:	Food and Waterborne Parasitology
Subjects:	Multiplex PCR Opisthorchiidae Heterophyidae Freshwater fish Fish products Europe
Online Access:	http://www.sciencedirect.com/science/article/pii/S240567662030010X

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spelling	doaj-a2c9a29347274dbfb7123ad173b7fc622020-11-25T03:21:55ZengElsevierFood and Waterborne Parasitology2405-67662020-06-0119Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish productsMonica Caffara0Andrea Gustinelli1Angelica Mazzone2Maria L. Fioravanti3Corresponding author.; Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), ItalyAmong others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU project. Due to the small size of the metacercariae, the infective stage for human, these parasites cannot be detected visually in fish and monitoring requires expert application of time-consuming techniques. The aim of this was to develop a rapid and affordable molecular method based on multiplex PCR for simultaneous identification of metacercariae of the most common European Opisthorchiid and Heterophyid in fish or fish products.http://www.sciencedirect.com/science/article/pii/S240567662030010XMultiplex PCROpisthorchiidaeHeterophyidaeFreshwater fishFish productsEurope
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Monica Caffara Andrea Gustinelli Angelica Mazzone Maria L. Fioravanti
spellingShingle	Monica Caffara Andrea Gustinelli Angelica Mazzone Maria L. Fioravanti Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products Food and Waterborne Parasitology Multiplex PCR Opisthorchiidae Heterophyidae Freshwater fish Fish products Europe
author_facet	Monica Caffara Andrea Gustinelli Angelica Mazzone Maria L. Fioravanti
author_sort	Monica Caffara
title	Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_short	Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_full	Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_fullStr	Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_full_unstemmed	Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products
title_sort	multiplex pcr for simultaneous identification of the most common european opisthorchiid and heterophyid in fish or fish products
publisher	Elsevier
series	Food and Waterborne Parasitology
issn	2405-6766
publishDate	2020-06-01
description	Among others, the families Opisthorchiidae and Heterophyidae includes several genera causing fish-borne zoonoses and distributed also in European Countries and that are included in the ParaFishControl (Advanced Tools and Research Strategies for Parasite Control in European farmed fish) H2020 EU project. Due to the small size of the metacercariae, the infective stage for human, these parasites cannot be detected visually in fish and monitoring requires expert application of time-consuming techniques. The aim of this was to develop a rapid and affordable molecular method based on multiplex PCR for simultaneous identification of metacercariae of the most common European Opisthorchiid and Heterophyid in fish or fish products.
topic	Multiplex PCR Opisthorchiidae Heterophyidae Freshwater fish Fish products Europe
url	http://www.sciencedirect.com/science/article/pii/S240567662030010X
work_keys_str_mv	AT monicacaffara multiplexpcrforsimultaneousidentificationofthemostcommoneuropeanopisthorchiidandheterophyidinfishorfishproducts AT andreagustinelli multiplexpcrforsimultaneousidentificationofthemostcommoneuropeanopisthorchiidandheterophyidinfishorfishproducts AT angelicamazzone multiplexpcrforsimultaneousidentificationofthemostcommoneuropeanopisthorchiidandheterophyidinfishorfishproducts AT marialfioravanti multiplexpcrforsimultaneousidentificationofthemostcommoneuropeanopisthorchiidandheterophyidinfishorfishproducts
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Multiplex PCR for simultaneous identification of the most common European Opisthorchiid and Heterophyid in fish or fish products

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