Toxicological evaluation of the biflavonoid, agathisflavone in albino Swiss mice

• Main Authors: Anderson Wilbur Lopes Andrade, Daiana Dias Ribeiro Figueiredo, Muhammad Torequl Islam, Adriana Maria Viana Nunes, Keylla da Conceição Machado, Katia da Conceição Machado, Shaikh Jamal Uddin, Jamil Ahmed Shilpi, Razina Rouf, Ana Amélia de Carvalho Melo-Cavalcante, Jorge Mauricio David, Mohammad S. Mubarak, Jéssica Pereira Costa
• Format: Article
• Language: English
• Published: Elsevier 2019-02-01
• Series: Biomedicine & Pharmacotherapy
• Subjects: Agathisflavone; Anacardium occidentale; Toxicological assessment; Open field test
• Online Access: http://www.sciencedirect.com/science/article/pii/S0753332218365272

Agathisflavone (AGF) is a biflavonoid with a number of important biological and pharmacological activities, such as antioxidant, antimicrobial, and neuroprotective effects. However, its toxicological effects have not been fully investigated. Accordingly, the aim of this study was to investigate the toxicological effects of AGF in mice. For this purpose, the median lethal dose 50% (LD50) was determined along with the anatomic and histopathological parameters (weight, alimentation, excretion, biochemical, and hematological) in fertile untouched female Swiss mice. Results suggest that during the treatment, no deaths were reported at 300 and 2000 mg/kg (n = 03/group, p.o.). Moreover, AGF did not cause significant change in the above mentioned parameters in test animals when compared with the control group (0.05% Tween 80 dissolved in 0.9% saline). Taken all together, this non-clinical toxicological study revealed that AGF has an LD50 larger than 2000 mg/kg and did not change significantly the hematological, biochemical, histopathological, behavioral, as well as physiological parameters in the female mice.