Identification of Pyoverdine Gene in the Human and Animal in Pseudomonas aeruginosa Isolates by Multiplex-PCR Method

• Main Authors: Elham Ranjbar, Kumarss Amini
• Format: Article
• Language: fas
• Published: Tehran University of Medical Sciences 2018-03-01
• Series: پیاورد سلامت
• Subjects: pseudomonas aeruginosa; pyoverdine; pcr multiple
• Online Access: http://payavard.tums.ac.ir/article-1-6429-en.html

Background and Aim: Pseudomonas aeruginosa is one of the most important factors in hospital infections, especially in patients with immune deficiency and Childhood diseases. The Virulence of bacteria are due to the presence of the Pyoverdine gene, which has many effects on the wild type of bacteria during the pathogenic pathway. Identification of different classes of PVD gene is necessary for the development of prevention and control Diseases program. In this research, the presence of PVD genes in the samples and their effect on pathogenicity was isolated and investigated. Materials and Methods: In the present study 60 Species of P. aeruginosa was isolated from clinical samples of human and animal, after approval by diagnostic tests and differential, were studied. Finally, for every 60 Species, isolated, Multiplex PCR was performed to detect target genes. Multiplex PCR method is to be considered as the gold standard. Its results are more reliable. Results: The results showed that the frequency of PVD gene in human isolates and livestock gene encoding the 3rd Pyoverdine was the highest frequency with 76.6% the lowest frequency is for Pyoverdine class 2 with 46%. Conclusion: As a result, the identification of three type’s genes of PVD classes in all strains tested by Pseudomonas could help to identify human patients and livestock with Pseudomonas infection and, given the presence of the gene encoding PVDs, has a direct relation to important bacterial pathogenicity.