Imaging the electrical activity of organelles in living cells

Matamala et al. adapt a hybrid-FRET voltage sensor to enable the recording of resting membrane potential of different organelle compartments in living cells. Their approach allows simple and precise subcellular targeting, the ability to record from individual organelles, and the potential for optica...

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Main Authors:	Ella Matamala, Cristian Castillo, Juan P. Vivar, Patricio A. Rojas, Sebastian E. Brauchi
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-03-01
Series:	Communications Biology
Online Access:	https://doi.org/10.1038/s42003-021-01916-6

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spelling	doaj-a2aa437d0f974fd88ef1cb32dd16520c2021-03-28T11:24:13ZengNature Publishing GroupCommunications Biology2399-36422021-03-014111210.1038/s42003-021-01916-6Imaging the electrical activity of organelles in living cellsElla Matamala0Cristian Castillo1Juan P. Vivar2Patricio A. Rojas3Sebastian E. Brauchi4Physiology Institute, Universidad Austral de ChileMillennium Nucleus of Ion Channel-Associated Diseases (MiNICAD)Physiology Institute, Universidad Austral de ChileLaboratory of Neuroscience, Faculty of Chemistry and Biology, Universidad de Santiago de ChilePhysiology Institute, Universidad Austral de ChileMatamala et al. adapt a hybrid-FRET voltage sensor to enable the recording of resting membrane potential of different organelle compartments in living cells. Their approach allows simple and precise subcellular targeting, the ability to record from individual organelles, and the potential for optical multiplexing of organellar activity.https://doi.org/10.1038/s42003-021-01916-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ella Matamala Cristian Castillo Juan P. Vivar Patricio A. Rojas Sebastian E. Brauchi
spellingShingle	Ella Matamala Cristian Castillo Juan P. Vivar Patricio A. Rojas Sebastian E. Brauchi Imaging the electrical activity of organelles in living cells Communications Biology
author_facet	Ella Matamala Cristian Castillo Juan P. Vivar Patricio A. Rojas Sebastian E. Brauchi
author_sort	Ella Matamala
title	Imaging the electrical activity of organelles in living cells
title_short	Imaging the electrical activity of organelles in living cells
title_full	Imaging the electrical activity of organelles in living cells
title_fullStr	Imaging the electrical activity of organelles in living cells
title_full_unstemmed	Imaging the electrical activity of organelles in living cells
title_sort	imaging the electrical activity of organelles in living cells
publisher	Nature Publishing Group
series	Communications Biology
issn	2399-3642
publishDate	2021-03-01
description	Matamala et al. adapt a hybrid-FRET voltage sensor to enable the recording of resting membrane potential of different organelle compartments in living cells. Their approach allows simple and precise subcellular targeting, the ability to record from individual organelles, and the potential for optical multiplexing of organellar activity.
url	https://doi.org/10.1038/s42003-021-01916-6
work_keys_str_mv	AT ellamatamala imagingtheelectricalactivityoforganellesinlivingcells AT cristiancastillo imagingtheelectricalactivityoforganellesinlivingcells AT juanpvivar imagingtheelectricalactivityoforganellesinlivingcells AT patricioarojas imagingtheelectricalactivityoforganellesinlivingcells AT sebastianebrauchi imagingtheelectricalactivityoforganellesinlivingcells
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Imaging the electrical activity of organelles in living cells

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