Clustered gene expression changes flank targeted gene loci in knockout mice.

Clustered gene expression changes flank targeted gene loci in knockout mice.

Gene expression profiling using microarrays is a powerful technology widely used to study regulatory networks. Profiling of mRNA levels in mutant organisms has the potential to identify genes regulated by the mutated protein.Using tissues from multiple lines of knockout mice we have examined genome-...

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Main Authors: Luis M Valor, Seth G N Grant
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-12-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2110885?pdf=render
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spelling doaj-a2892cb2fb234aecb20eba191cd101822020-11-24T20:40:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-12-01212e130310.1371/journal.pone.0001303Clustered gene expression changes flank targeted gene loci in knockout mice.Luis M ValorSeth G N GrantGene expression profiling using microarrays is a powerful technology widely used to study regulatory networks. Profiling of mRNA levels in mutant organisms has the potential to identify genes regulated by the mutated protein.Using tissues from multiple lines of knockout mice we have examined genome-wide changes in gene expression. We report that a significant proportion of changed genes were found near the targeted gene.The apparent clustering of these genes was explained by the presence of flanking DNA from the parental ES cell. We provide recommendations for the analysis and reporting of microarray data from knockout mice.http://europepmc.org/articles/PMC2110885?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Luis M Valor
Seth G N Grant
spellingShingle Luis M Valor
Seth G N Grant
Clustered gene expression changes flank targeted gene loci in knockout mice.
PLoS ONE
author_facet Luis M Valor
Seth G N Grant
author_sort Luis M Valor
title Clustered gene expression changes flank targeted gene loci in knockout mice.
title_short Clustered gene expression changes flank targeted gene loci in knockout mice.
title_full Clustered gene expression changes flank targeted gene loci in knockout mice.
title_fullStr Clustered gene expression changes flank targeted gene loci in knockout mice.
title_full_unstemmed Clustered gene expression changes flank targeted gene loci in knockout mice.
title_sort clustered gene expression changes flank targeted gene loci in knockout mice.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2007-12-01
description Gene expression profiling using microarrays is a powerful technology widely used to study regulatory networks. Profiling of mRNA levels in mutant organisms has the potential to identify genes regulated by the mutated protein.Using tissues from multiple lines of knockout mice we have examined genome-wide changes in gene expression. We report that a significant proportion of changed genes were found near the targeted gene.The apparent clustering of these genes was explained by the presence of flanking DNA from the parental ES cell. We provide recommendations for the analysis and reporting of microarray data from knockout mice.
url http://europepmc.org/articles/PMC2110885?pdf=render
work_keys_str_mv AT luismvalor clusteredgeneexpressionchangesflanktargetedgenelociinknockoutmice
AT sethgngrant clusteredgeneexpressionchangesflanktargetedgenelociinknockoutmice
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