Systematic analysis of mRNA 5' coding sequence incompleteness in <it>Danio rerio</it>: an automated EST-based approach

<p>Abstract</p> <p>Background</p> <p>All standard methods for cDNA cloning are affected by a potential inability to effectively clone the 5' region of mRNA. The aim of this work was to estimate mRNA open reading frame (ORF) 5' region sequence completeness in t...

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Bibliographic Details
Main Authors: Zannotti Maria, Carinci Paolo, Facchin Federica, Vitale Lorenza, Canaider Silvia, Lenzi Luca, Casadei Raffaella, Frabetti Flavia, Strippoli Pierluigi
Format: Article
Language:English
Published: BMC 2007-11-01
Series:Biology Direct
Online Access:http://www.biology-direct.com/content/2/1/34