| Summary: | Qiaocheng Chang1,*, Xiaodong Sun2,*, Jian Wang2,*, Jigang Yin1, Junpeng Song1, Shuai Peng1, Huijun Lu1, Hongning Zhou2, Ning Jiang1, Qijun Chen1,31Key Laboratory of Zoonosis, Jilin University, Changchun; 2Institute for Parasitic Disease Control of Yunnan Province, Puer City, Yunnan; 3Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China *These authors contributed equally to this workBackground: Long-tailed and pig-tailed macaque monkeys are natural hosts of Plasmodium knowlesi, which has been identified as a fifth malaria parasite infecting humans. In this study, we investigated possible infection by this Plasmodium parasite in macaque monkeys using a combination of polymerase chain reaction amplification and sequencing.Methods: Forty-five blood samples were obtained in 2010 from macaques in northern Myanmar near Yunnan Province of China and investigated for possible infection with Plasmodium species using a nested polymerase chain reaction method for amplification of 18S SSU rRNA genes.Results: Positive amplification was obtained from one monkey, and both sequence and phylogenetic analysis indicated that the parasite was of the Hepatocystis species lineage.Conclusion: The results suggest that a combination of polymerase chain reaction amplification and sequence identification would be necessary for detection of Plasmodium knowlesi infection in both humans and its natural hosts.Keywords: Plasmodium knowlesi, monkey, parasite, malaria
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