Data set for diet specific differential gene expression analysis in three Spodoptera moths

Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores, Spodoptera spp (S. littoralis, S. frugiperda maize (C) and rice (R) strains) for a RNAseq analysis of the midguts fr...

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Main Authors: A. Roy, W.B. Walker, III, H. Vogel, S.K. Kushwaha, S. Chattington, M.C. Larsson, P. Anderson, D.G. Heckel, F. Schlyter
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916302438
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spelling doaj-a1f372da701c4848a05b3c1107662e5f2020-11-25T01:51:49ZengElsevierData in Brief2352-34092016-09-018448455Data set for diet specific differential gene expression analysis in three Spodoptera mothsA. Roy0W.B. Walker, III1H. Vogel2S.K. Kushwaha3S. Chattington4M.C. Larsson5P. Anderson6D.G. Heckel7F. Schlyter8Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenChemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden; Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745 Jena, GermanyDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745 Jena, GermanyDepartment of Plant Breeding, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenChemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenChemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenChemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745 Jena, GermanyChemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden; Corresponding author.Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores, Spodoptera spp (S. littoralis, S. frugiperda maize (C) and rice (R) strains) for a RNAseq analysis of the midguts from the 3rd instar insect larvae for differential metabolic responses after feeding on pinto bean based artificial diet vs maize leaves. Paired-end (2×100 bp) Illumina HiSeq2500 sequencing resulted in a total of 24, 23, 24, and 21 million reads for the SF-C-Maize, SF-C-Pinto, SF-R-Maize, SF-R Pinto, and a total of 35 and 36 million reads for the SL-Maize and SL-Pinto samples, respectively. After quality control measures, a total of 62.2 million reads from SL and 71.7 million reads from SF were used for transcriptome assembly (TA). The resulting final de novo reference TA (backbone) for the SF taxa contained 37,985 contigs with a N50 contig size of 1030 bp and a maximum contig length of 17,093 bp, while for SL, 28,329 contigs were generated with a N50 contig size of 1980 bp and a maximum contig length of 18,267 bp. The data presented herein contains supporting information related to our research article Roy et al. (2016) http://dx.doi.org/10.1016/j.ibmb.2016.02.006 [1]. Keywords: Differential expression analysis (DGE), Transcriptomics, Spodoptera, Adaptation, Generalist, Specialist, RPKM (reads per kilo base of transcript per million mapped reads), RNA seqhttp://www.sciencedirect.com/science/article/pii/S2352340916302438
collection DOAJ
language English
format Article
sources DOAJ
author A. Roy
W.B. Walker, III
H. Vogel
S.K. Kushwaha
S. Chattington
M.C. Larsson
P. Anderson
D.G. Heckel
F. Schlyter
spellingShingle A. Roy
W.B. Walker, III
H. Vogel
S.K. Kushwaha
S. Chattington
M.C. Larsson
P. Anderson
D.G. Heckel
F. Schlyter
Data set for diet specific differential gene expression analysis in three Spodoptera moths
Data in Brief
author_facet A. Roy
W.B. Walker, III
H. Vogel
S.K. Kushwaha
S. Chattington
M.C. Larsson
P. Anderson
D.G. Heckel
F. Schlyter
author_sort A. Roy
title Data set for diet specific differential gene expression analysis in three Spodoptera moths
title_short Data set for diet specific differential gene expression analysis in three Spodoptera moths
title_full Data set for diet specific differential gene expression analysis in three Spodoptera moths
title_fullStr Data set for diet specific differential gene expression analysis in three Spodoptera moths
title_full_unstemmed Data set for diet specific differential gene expression analysis in three Spodoptera moths
title_sort data set for diet specific differential gene expression analysis in three spodoptera moths
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-09-01
description Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores, Spodoptera spp (S. littoralis, S. frugiperda maize (C) and rice (R) strains) for a RNAseq analysis of the midguts from the 3rd instar insect larvae for differential metabolic responses after feeding on pinto bean based artificial diet vs maize leaves. Paired-end (2×100 bp) Illumina HiSeq2500 sequencing resulted in a total of 24, 23, 24, and 21 million reads for the SF-C-Maize, SF-C-Pinto, SF-R-Maize, SF-R Pinto, and a total of 35 and 36 million reads for the SL-Maize and SL-Pinto samples, respectively. After quality control measures, a total of 62.2 million reads from SL and 71.7 million reads from SF were used for transcriptome assembly (TA). The resulting final de novo reference TA (backbone) for the SF taxa contained 37,985 contigs with a N50 contig size of 1030 bp and a maximum contig length of 17,093 bp, while for SL, 28,329 contigs were generated with a N50 contig size of 1980 bp and a maximum contig length of 18,267 bp. The data presented herein contains supporting information related to our research article Roy et al. (2016) http://dx.doi.org/10.1016/j.ibmb.2016.02.006 [1]. Keywords: Differential expression analysis (DGE), Transcriptomics, Spodoptera, Adaptation, Generalist, Specialist, RPKM (reads per kilo base of transcript per million mapped reads), RNA seq
url http://www.sciencedirect.com/science/article/pii/S2352340916302438
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