High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone that has profound effects on treatment of metabolic diseases. However, rhFGF21 is prone to form inclusion body when expressed in bacteria, which results in, the downstream process of purification of b...

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Bibliographic Details
Main Authors: Dandan Li, Gang Fu, Ran Tu, Zhaoxia Jin, Dawei Zhang
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Microbial Cell Factories
Subjects:
FGF21
Bacillus subtilis
Heterologous protein expression
Mini-cistron
Chaperone
Signal peptide
Online Access:http://link.springer.com/article/10.1186/s12934-019-1066-4

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http://link.springer.com/article/10.1186/s12934-019-1066-4

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