Isolation and Proto-cooperation of Pseudomonas putida TS 18 from Water Calyx Fluid of Spathodea campanulata P. Beauv.

• Main Authors: Martin Paul A., Jayanthi D., Thamizhseran N.
• Format: Article
• Language: English
• Published: Journal of Pure and Applied Microbiology 2019-12-01
• Series: Journal of Pure and Applied Microbiology
• Subjects: water calyces; p. putida; 16s rdna sequencing
• Online Access: https://microbiologyjournal.org/isolation-and-proto-cooperation-of-pseudomonas-putida-ts-18-from-water-calyx-fluid-of-spathodea-campanulata-p-beauv/

The study was to isolate, characterize and identify bacteria present in the water calyx fluid of the African tulip, Spathodea campanulata P. Beauv. Nutrient agar and nitrogen free malate (Nfb) semi-solid media were used to isolate the bacteria. Bacteria was identified using 16S rDNA sequencing. Pseudomonas putida TS 18 was found to be a new strain based on highest homology during the BLAST. Morphological and biochemical characterization of the identified bacteria was done by routine methods. This is the first study to report bacteria from the water calyx fluid of S. campanulata. Identified bacteria along with fluid content might be playing a unique role in the flower development by showing nitrogen fixing potential under in vitro conditions.