A Revised Protocol for Culture of Airway Epithelial Cells as a Diagnostic Tool for Primary Ciliary Dyskinesia

A Revised Protocol for Culture of Airway Epithelial Cells as a Diagnostic Tool for Primary Ciliary Dyskinesia

Air–liquid interface (ALI) culture of nasal epithelial cells is a valuable tool in the diagnosis and research of primary ciliary dyskinesia (PCD). Ex vivo samples often display secondary dyskinesia from cell damage during sampling, infection or inflammation confounding PCD diagnostic results. ALI cu...

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Bibliographic Details
Main Authors: Janice L. Coles, James Thompson, Katie L. Horton, Robert A. Hirst, Paul Griffin, Gwyneth M. Williams, Patricia Goggin, Regan Doherty, Peter M. Lackie, Amanda Harris, Woolf T. Walker, Christopher O’Callaghan, Claire Hogg, Jane S. Lucas, Cornelia Blume, Claire L. Jackson
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Journal of Clinical Medicine
Subjects:
PCD
ALI culture
bio-resource
primary nasal epithelium
diagnostics
Online Access:https://www.mdpi.com/2077-0383/9/11/3753

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