Purification and partial characterization of sphingomyelin from human plasma*

Purification and partial characterization of sphingomyelin from human plasma*

Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic...

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Main Author: Charles C. Sweeley
Format: Article
Language:English
Published: Elsevier 1963-10-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520402822
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spelling doaj-a1a3b686ab2a48999d177794e6496e9d2021-04-23T06:12:32ZengElsevierJournal of Lipid Research0022-22751963-10-0144402406Purification and partial characterization of sphingomyelin from human plasma*Charles C. Sweeley0Department of Biochemistry and Nutrition, Graduate School of Public Health, University of Pittsburgh, Pittsburgh 13, Pa.Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic acids as well. Palmitic acid is the predominant component; seven of the acids constitute over 90% of the total fraction. A previously unassigned trace constituent of the fatty acid fraction has been tentatively identified as a C24 dienoic acid on the basis of relative retention times, microhydrogenation, and reaction with mercuric acetate.http://www.sciencedirect.com/science/article/pii/S0022227520402822
collection DOAJ
language English
format Article
sources DOAJ
author Charles C. Sweeley
spellingShingle Charles C. Sweeley
Purification and partial characterization of sphingomyelin from human plasma*
Journal of Lipid Research
author_facet Charles C. Sweeley
author_sort Charles C. Sweeley
title Purification and partial characterization of sphingomyelin from human plasma*
title_short Purification and partial characterization of sphingomyelin from human plasma*
title_full Purification and partial characterization of sphingomyelin from human plasma*
title_fullStr Purification and partial characterization of sphingomyelin from human plasma*
title_full_unstemmed Purification and partial characterization of sphingomyelin from human plasma*
title_sort purification and partial characterization of sphingomyelin from human plasma*
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1963-10-01
description Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic acids as well. Palmitic acid is the predominant component; seven of the acids constitute over 90% of the total fraction. A previously unassigned trace constituent of the fatty acid fraction has been tentatively identified as a C24 dienoic acid on the basis of relative retention times, microhydrogenation, and reaction with mercuric acetate.
url http://www.sciencedirect.com/science/article/pii/S0022227520402822
work_keys_str_mv AT charlescsweeley purificationandpartialcharacterizationofsphingomyelinfromhumanplasma
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