Purification and partial characterization of sphingomyelin from human plasma*
Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic...
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| Format: | Article |
| Language: | English |
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Elsevier
1963-10-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520402822 |
| Summary: | Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic acids as well. Palmitic acid is the predominant component; seven of the acids constitute over 90% of the total fraction. A previously unassigned trace constituent of the fatty acid fraction has been tentatively identified as a C24 dienoic acid on the basis of relative retention times, microhydrogenation, and reaction with mercuric acetate. |
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| ISSN: | 0022-2275 |