Variation in genomic islands contribute to genome plasticity in <it>cupriavidus metallidurans</it>

• Main Authors: Van Houdt Rob, Monsieurs Pieter, Mijnendonckx Kristel, Provoost Ann, Janssen Ann, Mergeay Max, Leys Natalie
• Format: Article
• Language: English
• Published: BMC 2012-03-01
• Series: BMC Genomics
• Online Access: http://www.biomedcentral.com/1471-2164/13/111

<p>Abstract</p> <p>Background</p> <p>Different <it>Cupriavidus metallidurans </it>strains isolated from metal-contaminated and other anthropogenic environments were genotypically and phenotypically compared with <it>C. metallidurans </it>type strain CH34. The latter is well-studied for its resistance to a wide range of metals, which is carried for a substantial part by its two megaplasmids pMOL28 and pMOL30.</p> <p>Results</p> <p>Comparative genomic hybridization (CGH) indicated that the extensive arsenal of determinants involved in metal resistance was well conserved among the different <it>C. metallidurans </it>strains. Contrary, the mobile genetic elements identified in type strain CH34 were not present in all strains but clearly showed a pattern, although, not directly related to a particular biotope nor location (geographical). One group of strains carried almost all mobile genetic elements, while these were much less abundant in the second group. This occurrence was also reflected in their ability to degrade toluene and grow autotrophically on hydrogen gas and carbon dioxide, which are two traits linked to separate genomic islands of the Tn<it>4371</it>-family. In addition, the clear pattern of genomic islands distribution allowed to identify new putative genomic islands on chromosome 1 and 2 of <it>C. metallidurans </it>CH34.</p> <p>Conclusions</p> <p>Metal resistance determinants are shared by all <it>C. metallidurans </it>strains and their occurrence is apparently irrespective of the strain's isolation type and place. <it>Cupriavidus metallidurans </it>strains do display substantial differences in the diversity and size of their mobile gene pool, which may be extensive in some (including the type strain) while marginal in others.</p>