Multiplikasi, Induksi Planlet dan Seleksi Tembakau Hasil Transformasi Gen Coat Protein SMV Secara Kultur in Vitro

• Main Authors: N.A. FITRIYAH, I.A. RINEKSANE, E. HANDAYANI, AGUNG-ASTUTI
• Format: Article
• Language: English
• Published: MBI & UNS Solo 2004-11-01
• Series: Bioteknologi
• Subjects: multiplication; induction; selection; tobacco; SMV coat protein gene
• Online Access: http://biosains.mipa.uns.ac.id/C/C0102/C010201.pdf

A study has been conducted to screen and multiply calluses and buds oftobacco transformed with the Soybean Mosaic Virus (SMV) coat protein gene and to induce them to plantlet formation by using MS medium with phytohormon treatments. The multiplication of calluses and buds was carried out by sub-culturing on MS medium using 0.3 mg/L NAA+1 mg/L BAP, followed by induction of plantlet formation on MS medium supplemented with NAA and BAP at varied concentration. The results showed that tobacco calluses transformed with SMV coat protein gene was multipliable on MS medium supplemented with NAA at a concentration of 0.3 mg/mL and BAP at 1 mg/L concentration. In this experiment kanamycin was used at a concentration of 100 μg/mL which resulted in the viability level of 84%. On MS medium supplemented with 0.3 mg/L NAA, 0.1 mg/L BAP, and 100 μg/mL kanamycin, induction of calluses to plantlet formation reached 20% level.