Summary: | This study examined the correlation between non-metallic organic tanning agents (NOTAs) and wastewater containing double-triazine (bistriazine). Specifically, high-throughput sequencing was used to analyze how the physical and chemical properties of the bistriazine-containing wastewater affected the microbial community. In total, 9995 operating taxonomy units (OTUs) were detected at a similarity level of 97%. According to the taxonomy results and relative bacterial richness, the non-metallic organic tanning agent (NOTA) wastewater communities showed significant differences. <i>Clostridium sensu stricto</i> and <i>Turicibacter</i> bacteria were dominant in NOTA T2, T3, and the blank control (KB) wastewater; <i>Trichococcus</i> and <i>Aeromonas</i> were dominant in NOTA T4; and <i>Pseudomonas</i> was dominant in T2, T4, and the blank control (KB). Thus, the addition of bistriazine ring derivatives altered the composition of the bacterial community. Furthermore, Spearman’s correlation analysis revealed a substantial correlation in the composition of the communities and the physicochemical properties of the wastewater. <i>Acidocella</i> and <i>norank c Cyanobacteria</i> correlated positively with Total Phosphorus (TP) (<i>p</i> ≤ 0.001) and <i>Mycobacterium</i> showed a positively held correlation with pH (<i>p</i> ≤ 0.05). Conversely, <i>norank_f__Elev-16S-1332</i> indicated a negative correlation with TP, Total Nitrogen (TN), and Total Organic Carbon (TOC) (<i>p</i> ≤ 0.05)<i>,</i> whereas <i>Zoogloea</i> correlated negatively with NH<sub>3</sub>–N, TOC, and TN (<i>p</i> ≤ 0.05). Regarding water quality, a significant correlation between microbial communities and the physicochemical properties of bistriazine wastewater was also observed (<i>p</i> ≤ 0.05). We concluded that <i>Trichococcus</i>, <i>Clostridium sensu stricto</i>, <i>Turicibacter</i>, <i>Pseudomonas</i>, <i>Intestinibacter</i>, <i>Acidocella</i>, <i>Bacillus</i>, and <i>Tolumonas</i> showed a high tolerance for bistriazine wastewater, which could offer insight into its bioremediation.
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