Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Abstract Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from...

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Bibliographic Details
Main Authors: Rebecca L. Vanders, Alan Hsu, Peter G. Gibson, Vanessa E. Murphy, Peter A. B. Wark
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Respiratory Research
Subjects:
Pregnancy
Asthma
Nasal cells
Respiratory infections
Influenza
Rhinovirus
Online Access:http://link.springer.com/article/10.1186/s12931-019-1225-5
Description
Summary:Abstract Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.
ISSN:1465-993X

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