DETECTION OF SRY OF NEWBORN RABBITS MENT FOR XENOIMPLANTATES

• Main Authors: ALICA RAFAYOVÁ, ANNA TRAKOVICKÁ, V. PARKÁNYI, A. KOVÁČIK
• Format: Article
• Language: English
• Published: Agroprint Timisoara 2009-10-01
• Series: Scientific Papers Animal Science and Biotechnologies
• Subjects: sex – detection; sry; rabbit
• Online Access: http://spasb.ro/index.php/spasb/article/view/1105

The aim of this study was sex-detection of nexborn rabbits with molecular-genetics analyses of SRY (specific region of Y chromosome) sequence. This region is present only in male animals, in females is missing. We analysed 16 newborn rabbits (native in two litters of females lines P91 and M91) by using polymerase chain reaction with specific primers. PCR product was visualised at 3% agarose gel. SRY – PCR fragment (242 bp) was detected in 8 animals, 8 animals had no fragment present. All young were evaluated according to outer genitals at 3 weeks of age. This technology provides partly selective tool for categorization of the females and males in further reproductive process. Both sexes of newborn rabbits can be used for control of production of special enzymes under genetic control of the gonosomes and also in human medicine for aplication of xenoimplatates.