Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
Background and objectives: Pyrococcus woesei is a hyperthermophilic archaea and produces a heat stable polymerase(Pwo polymerase) that has proofreading activity. Materials and Methods: In this study, this microorganism was cultured, its DNA was extracted and the pwo gene polymerase was cloned, expr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2011-09-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/99 |