Frequency of Helicobacter pylori blood-group antigen-binding adhesion 2 and sialic acid binding adhesion genes among dyspeptic patients in Tabriz, Iran

• Main Authors: Leila Yousefi, Reza Ghotaslou, Mohammad Taghi Akhi, Mohammad Asgharzadeh, Mohammad Reza Nahaei, Mohammad Hosein Somi, Mandana Rafeey
• Format: Article
• Language: English
• Published: Tabriz University of Medical Sciences 2015-06-01
• Series: Journal of Analytical Research in Clinical Medicine
• Subjects: Blood-group Antigen-binding Adhesion 2 Gene; Sialic Acid Binding; Adhesion Gene; Helicobacter Pylori
• Online Access: http://journals.tbzmed.ac.ir/JARCM/Manuscript/JARCM-3-71.pdf

Introduction: The purpose of this research was to analyze blood-group antigen-binding adhesion (babA2) and sialic acid binding adhesion (sabA) genotypes status in Helicobacter pylori (H. pylori) isolates and their relationship with clinical outcomes. Methods: Gastric biopsy specimens were homogenized and placed in Brucella agar medium supplemented with 5% sheep blood and 3 antibiotics and were cultured at 37 °C under microaerophilic conditions and incubated for 4-7 days. H. pylori was identified by typical morphology, gram-staining and urease tests, and babA2 and sabA genes were detected by polymerase chain reaction (PCR). Results: From a total of 100 H. pylori isolates; babA2 and sabA genes were detected in 23.0 and 26.4%, respectively. There was a significant relationship between these genes and clinical outcomes (P < 0.050). Conclusion: We found that the babA2 status was not related to clinical outcomes in Tabriz, Iran. However, sabA was a promoting determinant for disease, and multivariate analysis disclosed sabA to be an independent marker of non-ulcer diseases in our subjects.