Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy

Microsphere-assisted nanoscopy has shown great potential in recent developments in the field of super-resolution imaging. The precise control of microspheres is leading to new discoveries that can help verify the theories behind the super-resolution imaging mechanism. However, microsphere imaging in...

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Main Authors: Boliang Jia, Pan Li, Feifei Wang, Ho Yin Chan, Guanglie Zhang, Wen Jung Li
Format: Article
Language:English
Published: IEEE 2020-01-01
Series:IEEE Open Journal of Nanotechnology
Subjects:
Online Access:https://ieeexplore.ieee.org/document/9153924/
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spelling doaj-a03c977fde6a47eba51627d5b338dffa2021-03-29T18:59:58ZengIEEEIEEE Open Journal of Nanotechnology2644-12922020-01-011657610.1109/OJNANO.2020.30134319153924Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens NanoscopyBoliang Jia0https://orcid.org/0000-0001-6392-8832Pan Li1Feifei Wang2Ho Yin Chan3Guanglie Zhang4Wen Jung Li5https://orcid.org/0000-0001-9616-6213Department of Mechanical Engineering, City University of Hong Kong, Hong Kong, ChinaCollege of Mechanical and Electrical Engineering, Hohai University, Jiangsu, ChinaDepartment of Chemistry, Stanford University, Stanford, CA, USADepartment of Mechanical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical Engineering, City University of Hong Kong, Hong Kong, ChinaMicrosphere-assisted nanoscopy has shown great potential in recent developments in the field of super-resolution imaging. The precise control of microspheres is leading to new discoveries that can help verify the theories behind the super-resolution imaging mechanism. However, microsphere imaging involves multiple planes that have different magnification factors, which affect the determination of the overall resolution of the image. In this study, we present a flexible probe-lens assembly scheme that uses a barium titanate glass microsphere, as well as various scanning stages that can be used to freely investigate the sample surface and perform large-area super-resolution imaging (80 μm × 60 μm). The obtained resolution using this assembly under water immersion condition is 130 nm. By investigating the relationship between the magnification factors and the corresponding focus position of the different feature patterns, a remarkable difference in the focusing characteristics between arbitrary and periodic patterns was revealed. Results demonstrate the universality of the proposed method for the quantitative selection of the best focused plane and determination of the corresponding magnification factor and resolution of a microsphere virtual image for any feature pattern. The findings provide additional insights into the interpretation of arbitrary nanostructures through 3D optical imaging.https://ieeexplore.ieee.org/document/9153924/Super-resolution imagingdielectric microspherescanning superlens microscopyscanning probe–lens microscopymagnification factormicro-robotics
collection DOAJ
language English
format Article
sources DOAJ
author Boliang Jia
Pan Li
Feifei Wang
Ho Yin Chan
Guanglie Zhang
Wen Jung Li
spellingShingle Boliang Jia
Pan Li
Feifei Wang
Ho Yin Chan
Guanglie Zhang
Wen Jung Li
Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy
IEEE Open Journal of Nanotechnology
Super-resolution imaging
dielectric microsphere
scanning superlens microscopy
scanning probe–lens microscopy
magnification factor
micro-robotics
author_facet Boliang Jia
Pan Li
Feifei Wang
Ho Yin Chan
Guanglie Zhang
Wen Jung Li
author_sort Boliang Jia
title Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy
title_short Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy
title_full Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy
title_fullStr Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy
title_full_unstemmed Determination of Microsphere-Lens Magnification Using Micro-Robotic Scanning Superlens Nanoscopy
title_sort determination of microsphere-lens magnification using micro-robotic scanning superlens nanoscopy
publisher IEEE
series IEEE Open Journal of Nanotechnology
issn 2644-1292
publishDate 2020-01-01
description Microsphere-assisted nanoscopy has shown great potential in recent developments in the field of super-resolution imaging. The precise control of microspheres is leading to new discoveries that can help verify the theories behind the super-resolution imaging mechanism. However, microsphere imaging involves multiple planes that have different magnification factors, which affect the determination of the overall resolution of the image. In this study, we present a flexible probe-lens assembly scheme that uses a barium titanate glass microsphere, as well as various scanning stages that can be used to freely investigate the sample surface and perform large-area super-resolution imaging (80 μm × 60 μm). The obtained resolution using this assembly under water immersion condition is 130 nm. By investigating the relationship between the magnification factors and the corresponding focus position of the different feature patterns, a remarkable difference in the focusing characteristics between arbitrary and periodic patterns was revealed. Results demonstrate the universality of the proposed method for the quantitative selection of the best focused plane and determination of the corresponding magnification factor and resolution of a microsphere virtual image for any feature pattern. The findings provide additional insights into the interpretation of arbitrary nanostructures through 3D optical imaging.
topic Super-resolution imaging
dielectric microsphere
scanning superlens microscopy
scanning probe–lens microscopy
magnification factor
micro-robotics
url https://ieeexplore.ieee.org/document/9153924/
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