SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells

• Main Authors: Lai Yu-Heng, Cheng Jessica, Cheng Dongmei, Feasel Mattie E, Beste Kyle D, Peng Junmin, Nusrat Asma, Moreno Carlos S
• Format: Article
• Language: English
• Published: BMC 2011-11-01
• Series: BMC Cell Biology
• Online Access: http://www.biomedcentral.com/1471-2121/12/50

<p>Abstract</p> <p>Background</p> <p>SOX4 is a developmental transcription factor that is required for differentiation and proliferation in multiple tissues. SOX4 is overexpressed in many human malignancies, but the precise role of SOX4 in cancer progression is still not well understood. Thus, the identification of additional SOX4 binding partners is essential for elucidating the mechanism of SOX4-mediated effects in cancer progression.</p> <p>Results</p> <p>Here, we have adapted a one-step affinity purification method that enables rapid purification of SOX4 complexes via intracellular biotinylation of the amino-terminus of SOX4 to perform large-scale proteomics analysis. We have discovered that junction plakoglobin (JUP) interacts with SOX4 in both the cytosol and the nucleus and the interaction between SOX4 and plakoglobin is significantly increased when prostate and breast cancer cells are stimulated with WNT3A. Interactions between SOX4 and plakoglobin were further enhanced by the nuclear export inhibitor leptomycin B (LMB), suggesting that plakoglobin promotes nuclear export of SOX4. The SOX4-plakoglobin complex affected the expression of Wnt pathway target genes and SOX4 downstream targets, such as <it>AXIN2</it>, <it>DICER1</it>, and <it>DHX9</it>. In addition, SOX4 DNA binding activity to the promoters of <it>DICER1</it>, <it>AXIN2</it>, <it>DHX9 </it>and <it>SOX4 </it>itself was reduced by conditions that promote SOX4-plakoglobin complex formation. Conditions that enhanced SOX4-plakoglobin interactions resulted in reduced transcriptional activity of β-catenin luciferase reporters.</p> <p>Conclusions</p> <p>These data suggest that this newly identified interaction between SOX4 and plakoglobin is inhibitory and provides new insights into the role of SOX4 in key pathways in cell proliferation, development, and cancer progression.</p>