Molecular identification and genetic relationships among coffee species (Coffea L.) inferred from ISSR and SRAP marker analyses

• Main Authors: Mishra Kumar Manoj, Nishani Sandhyarani, Jayarama
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2011-01-01
• Series: Archives of Biological Sciences
• Subjects: Coffee species; genetic relationships; molecular identification; inter simple sequence repeats (ISSR); sequence related amplified polymorphism (SRAP)
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2011/0354-46641103667M.pdf

The identification and genetic relationships of 23 coffee species and one coffee-related species Canthium diccocum were studied using ISSR and SRAP markers. The average polymorphism information content of SRAP primers (0.81) was lower than ISSR primers (0.86), whereas the average resolving power of the SRAP primers (9.74) is higher than the ISSR primers (8.64). The genetic similarity among the species ranged from 0.30 to 0.89 using ISSR and 0.11 to 0.90 using SRAP marker systems. Based on marker analysis, all twenty three coffee species were clustered into two major groups. Both the markers amplified species-specific fragments and are useful in genetic diversity analysis of coffee.