5' long terminal repeat (LTR)-selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals
<p>Abstract</p> <p>We previously described selective hypermethylation of the 5'-long terminal repeat (LTR) of HTLV-1 provirus <it>in vivo </it>and <it>in vitro</it>. This prompted us to analyze CpG methylation of the two LTRs of the HIV provirus in chro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-10-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/3/1/69 |
| Summary: | <p>Abstract</p> <p>We previously described selective hypermethylation of the 5'-long terminal repeat (LTR) of HTLV-1 provirus <it>in vivo </it>and <it>in vitro</it>. This prompted us to analyze CpG methylation of the two LTRs of the HIV provirus in chronically infected cell lines. The results demonstrate selective hypermethylation of the 5' LTR of the HIV provirus in ACH-2 cells. Moreover, induction of viral gene expression by TNF-α resulted in demethylation of the 5'-LTR. These results suggest that selective epigenetic modification of the 5'LTR of the HIV-1 provirus may be an important mechanism by which proviral activity is suppressed.</p> |
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| ISSN: | 1742-4690 |