A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding

A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding

We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patien...

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Bibliographic Details
Main Authors: Manoel Barral-Netto, A. Barral
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1986-09-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
concanavalin
mononuclear cells
visceral leishmaniasis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300012
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spelling doaj-a0037a2dc7a54e10986d617cb82c5f2f2020-11-24T20:42:43ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601986-09-0181334334510.1590/S0074-02761986000300012A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this bindingManoel Barral-NettoA. BarralWe report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300012concanavalinmononuclear cellsvisceral leishmaniasis
collection DOAJ
language English
format Article
sources DOAJ
author Manoel Barral-Netto
A. Barral
spellingShingle Manoel Barral-Netto
A. Barral
A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
Memórias do Instituto Oswaldo Cruz.
concanavalin
mononuclear cells
visceral leishmaniasis
author_facet Manoel Barral-Netto
A. Barral
author_sort Manoel Barral-Netto
title A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_short A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_full A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_fullStr A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_full_unstemmed A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
title_sort simple method for assessing the binding of concanavalin a to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding
publisher Instituto Oswaldo Cruz, Ministério da Saúde
series Memórias do Instituto Oswaldo Cruz.
issn 0074-0276
1678-8060
publishDate 1986-09-01
description We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
topic concanavalin
mononuclear cells
visceral leishmaniasis
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300012
work_keys_str_mv AT manoelbarralnetto asimplemethodforassessingthebindingofconcanavalinatomononuclearcellsurfacesnointerferenceofvisceralleishmaniasisserumonthisbinding
AT abarral asimplemethodforassessingthebindingofconcanavalinatomononuclearcellsurfacesnointerferenceofvisceralleishmaniasisserumonthisbinding
AT manoelbarralnetto simplemethodforassessingthebindingofconcanavalinatomononuclearcellsurfacesnointerferenceofvisceralleishmaniasisserumonthisbinding
AT abarral simplemethodforassessingthebindingofconcanavalinatomononuclearcellsurfacesnointerferenceofvisceralleishmaniasisserumonthisbinding
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