Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of <i>Nannochloropsis...
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doaj-9ff18663681d48e9ab74228ad5e09e552020-11-25T03:45:02ZengMDPI AGEnergies1996-10732020-07-01133813381310.3390/en13153813Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from MicroalgaeShuai Zhang0Yuyong Hou1Zhiyong Liu2Xiang Ji3Di Wu4Weijie Wang5Dongyuan Zhang6Wenya Wang7Shulin Chen8Fangjian Chen9Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaTianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaTianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, ChinaSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, ChinaCollege of Life Science, North China University of Science and Technology, Tangshan 063210, ChinaTianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaCollege of Life Science and Techenology, Beijing University of Chemical Technology, Beijing 100029, ChinaDepartment of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USATianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaCurrently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of <i>Nannochloropsis oceanica</i> IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO<sub>4</sub> in a 16.4 mA·cm<sup>−2</sup> current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.https://www.mdpi.com/1996-1073/13/15/3813lipid extractionelectro-Fenton reactioncell wall disruptionmicroalgae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuai Zhang Yuyong Hou Zhiyong Liu Xiang Ji Di Wu Weijie Wang Dongyuan Zhang Wenya Wang Shulin Chen Fangjian Chen |
spellingShingle |
Shuai Zhang Yuyong Hou Zhiyong Liu Xiang Ji Di Wu Weijie Wang Dongyuan Zhang Wenya Wang Shulin Chen Fangjian Chen Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae Energies lipid extraction electro-Fenton reaction cell wall disruption microalgae |
author_facet |
Shuai Zhang Yuyong Hou Zhiyong Liu Xiang Ji Di Wu Weijie Wang Dongyuan Zhang Wenya Wang Shulin Chen Fangjian Chen |
author_sort |
Shuai Zhang |
title |
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae |
title_short |
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae |
title_full |
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae |
title_fullStr |
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae |
title_full_unstemmed |
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae |
title_sort |
electro-fenton based technique to enhance cell harvest and lipid extraction from microalgae |
publisher |
MDPI AG |
series |
Energies |
issn |
1996-1073 |
publishDate |
2020-07-01 |
description |
Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of <i>Nannochloropsis oceanica</i> IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO<sub>4</sub> in a 16.4 mA·cm<sup>−2</sup> current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation. |
topic |
lipid extraction electro-Fenton reaction cell wall disruption microalgae |
url |
https://www.mdpi.com/1996-1073/13/15/3813 |
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