Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae

Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of <i>Nannochloropsis...

Full description

Bibliographic Details
Main Authors: Shuai Zhang, Yuyong Hou, Zhiyong Liu, Xiang Ji, Di Wu, Weijie Wang, Dongyuan Zhang, Wenya Wang, Shulin Chen, Fangjian Chen
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Energies
Subjects:
Online Access:https://www.mdpi.com/1996-1073/13/15/3813
id doaj-9ff18663681d48e9ab74228ad5e09e55
record_format Article
spelling doaj-9ff18663681d48e9ab74228ad5e09e552020-11-25T03:45:02ZengMDPI AGEnergies1996-10732020-07-01133813381310.3390/en13153813Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from MicroalgaeShuai Zhang0Yuyong Hou1Zhiyong Liu2Xiang Ji3Di Wu4Weijie Wang5Dongyuan Zhang6Wenya Wang7Shulin Chen8Fangjian Chen9Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaTianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaTianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, ChinaSchool of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, ChinaCollege of Life Science, North China University of Science and Technology, Tangshan 063210, ChinaTianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaCollege of Life Science and Techenology, Beijing University of Chemical Technology, Beijing 100029, ChinaDepartment of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USATianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaCurrently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of <i>Nannochloropsis oceanica</i> IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO<sub>4</sub> in a 16.4 mA·cm<sup>−2</sup> current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.https://www.mdpi.com/1996-1073/13/15/3813lipid extractionelectro-Fenton reactioncell wall disruptionmicroalgae
collection DOAJ
language English
format Article
sources DOAJ
author Shuai Zhang
Yuyong Hou
Zhiyong Liu
Xiang Ji
Di Wu
Weijie Wang
Dongyuan Zhang
Wenya Wang
Shulin Chen
Fangjian Chen
spellingShingle Shuai Zhang
Yuyong Hou
Zhiyong Liu
Xiang Ji
Di Wu
Weijie Wang
Dongyuan Zhang
Wenya Wang
Shulin Chen
Fangjian Chen
Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
Energies
lipid extraction
electro-Fenton reaction
cell wall disruption
microalgae
author_facet Shuai Zhang
Yuyong Hou
Zhiyong Liu
Xiang Ji
Di Wu
Weijie Wang
Dongyuan Zhang
Wenya Wang
Shulin Chen
Fangjian Chen
author_sort Shuai Zhang
title Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
title_short Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
title_full Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
title_fullStr Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
title_full_unstemmed Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae
title_sort electro-fenton based technique to enhance cell harvest and lipid extraction from microalgae
publisher MDPI AG
series Energies
issn 1996-1073
publishDate 2020-07-01
description Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of <i>Nannochloropsis oceanica</i> IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO<sub>4</sub> in a 16.4 mA·cm<sup>−2</sup> current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.
topic lipid extraction
electro-Fenton reaction
cell wall disruption
microalgae
url https://www.mdpi.com/1996-1073/13/15/3813
work_keys_str_mv AT shuaizhang electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT yuyonghou electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT zhiyongliu electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT xiangji electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT diwu electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT weijiewang electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT dongyuanzhang electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT wenyawang electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT shulinchen electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
AT fangjianchen electrofentonbasedtechniquetoenhancecellharvestandlipidextractionfrommicroalgae
_version_ 1724511930087899136