A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in <i>Arabidopsis</i>

A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in <i>Arabidopsis</i>

The CRISPR/Cas9 system has been widely used for targeted genome editing in numerous plant species. In <i>Arabidopsis</i>, constitutive promoters usually result in a low efficiency of heritable mutation in the T1 generation. In this work, CRISPR/Cas9 gene editing efficiencies using differ...

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Bibliographic Details
Main Authors: Zhengyan Feng, Zhengjing Zhang, Kai Hua, Xifeng Gao, Yanfei Mao, Jose Ramon Botella, Jian-Kang Zhu
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Arabidopsis</i>
cell division-specific Cas9 system
CRISPR/Cas9
multiplex gene editing
Pol III promoter
Online Access:https://www.mdpi.com/1422-0067/19/12/3925

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