Inhibitory Effect of Aquatic and Alcoholic Extracts of Artemisia sieberi on Growth of Candida Albicans: An In Vitro Study

• Main Authors: Ali Azam Taheri, Issa Gholampourazizi, Masoud Hashemi Karoui, Leyla Farhadi, Karo Servatyari, Samaneh Rouhi
• Format: Article
• Language: fas
• Published: Qom University of Medical Sciences 2018-08-01
• Series: Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum
• Subjects: aqueous and alcoholic extracts; artemisia; candida albicans; in vitro techniques.
• Online Access: http://journal.muq.ac.ir/browse.php?a_code=A-10-1060-1&slc_lang=en&sid=1

Background and Objectives: Candida albicans (C. albicans) as an opportunistic pathogen, can causes cutaneous and mucosal infections. Antibiotic treatments for this fungus have multiple adverse effects and cause drug resistance. In this study, the inhibitory effect of Artemisia sieberi (A. sieberi), was investigated on growth of Candida albicans.   Methods: In this experimental study, aqueous and alcoholic extracts were prepared from A. sieberi using percolation method. Using disk diffusion method, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), were assessed to evaluate the antifungal effects of these extracts on C. albicans in vitro.   Results: C. albicans, was resistant to hydroalcoholic extracts in the disk diffusion method and grew around the disks containing the extracts. The MIC of aqueous, ethanolic, and methanolic extracts, were determined to be 6250, 3125, and 6250µg/ml, respectively, and MFC of these three extracts, were determined 12500, 6250, and 12500µg/ml, respectively. In the MIC and MFC method, ethanolic extracts had higher antifungal activity.   Conclusion: According to the results of this study, different extracts of A. sieberi affect C. albicans growth and prevent its growth. Thus, more