Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle

Three-dimensional (3D) bioprinting is a novel technology utilizing biocompatible materials, cells, drugs, etc. as basic microcomponents to form 3D artificial structures and is believed as a promising method for regenerative medicine. Droplet-based bioprinting can precisely generate microspheres and...

Full description

Bibliographic Details
Main Authors: Shanguo Zhang, Guiling Li, Jia Man, Song Zhang, Jianyong Li, Jianfeng Li, Donghai Li
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/11/7/681
id doaj-9f99bf21da8e4d749ec17a578bc5e4cf
record_format Article
spelling doaj-9f99bf21da8e4d749ec17a578bc5e4cf2020-11-25T02:35:57ZengMDPI AGMicromachines2072-666X2020-07-011168168110.3390/mi11070681Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting NozzleShanguo Zhang0Guiling Li1Jia Man2Song Zhang3Jianyong Li4Jianfeng Li5Donghai Li6Key Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, ChinaSchool of Medicine, Tsinghua University, Beijing 100084, ChinaKey Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, ChinaKey Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, ChinaKey Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, ChinaKey Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, ChinaAdvanced Medical Research Institute, Shandong University, Jinan 250012, ChinaThree-dimensional (3D) bioprinting is a novel technology utilizing biocompatible materials, cells, drugs, etc. as basic microcomponents to form 3D artificial structures and is believed as a promising method for regenerative medicine. Droplet-based bioprinting can precisely generate microspheres and manipulate them into organized structures with high fidelity. Biocompatible hydrogels are usually used as bioinks in 3D bioprinting, however, the viscosity of the bioink could be increased due to the additives such as cells, drugs, nutrient factors and other functional polymers in some particular applications, making it difficult to form monodispersed microspheres from high-viscosity bioink at the orifice of the nozzle. In this work, we reported a novel microfluidic-based printing nozzle to prepare monodispersed microspheres from high-viscosity bioink using the phase-inversion method. Different flowing conditions can be achieved by changing the flow rates of the fluids to form monodispersed solid and hollow microspheres using the same nozzle. The diameter of the microspheres can be tuned by changing the flow rate ratio and the size distribution of the microspheres is narrow. The prepared calcium alginate microspheres could also act as micro-carriers in drug delivery.https://www.mdpi.com/2072-666X/11/7/681droplet-based bioprintingmicrofluidic systemphase-inversion methodmicrocomponent
collection DOAJ
language English
format Article
sources DOAJ
author Shanguo Zhang
Guiling Li
Jia Man
Song Zhang
Jianyong Li
Jianfeng Li
Donghai Li
spellingShingle Shanguo Zhang
Guiling Li
Jia Man
Song Zhang
Jianyong Li
Jianfeng Li
Donghai Li
Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle
Micromachines
droplet-based bioprinting
microfluidic system
phase-inversion method
microcomponent
author_facet Shanguo Zhang
Guiling Li
Jia Man
Song Zhang
Jianyong Li
Jianfeng Li
Donghai Li
author_sort Shanguo Zhang
title Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle
title_short Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle
title_full Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle
title_fullStr Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle
title_full_unstemmed Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle
title_sort fabrication of microspheres from high-viscosity bioink using a novel microfluidic-based 3d bioprinting nozzle
publisher MDPI AG
series Micromachines
issn 2072-666X
publishDate 2020-07-01
description Three-dimensional (3D) bioprinting is a novel technology utilizing biocompatible materials, cells, drugs, etc. as basic microcomponents to form 3D artificial structures and is believed as a promising method for regenerative medicine. Droplet-based bioprinting can precisely generate microspheres and manipulate them into organized structures with high fidelity. Biocompatible hydrogels are usually used as bioinks in 3D bioprinting, however, the viscosity of the bioink could be increased due to the additives such as cells, drugs, nutrient factors and other functional polymers in some particular applications, making it difficult to form monodispersed microspheres from high-viscosity bioink at the orifice of the nozzle. In this work, we reported a novel microfluidic-based printing nozzle to prepare monodispersed microspheres from high-viscosity bioink using the phase-inversion method. Different flowing conditions can be achieved by changing the flow rates of the fluids to form monodispersed solid and hollow microspheres using the same nozzle. The diameter of the microspheres can be tuned by changing the flow rate ratio and the size distribution of the microspheres is narrow. The prepared calcium alginate microspheres could also act as micro-carriers in drug delivery.
topic droplet-based bioprinting
microfluidic system
phase-inversion method
microcomponent
url https://www.mdpi.com/2072-666X/11/7/681
work_keys_str_mv AT shanguozhang fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
AT guilingli fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
AT jiaman fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
AT songzhang fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
AT jianyongli fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
AT jianfengli fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
AT donghaili fabricationofmicrospheresfromhighviscositybioinkusinganovelmicrofluidicbased3dbioprintingnozzle
_version_ 1724802386982076416