Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem

Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem

Rumen is an interesting ecosystem for microbial exploration and their products. Isolation of the chitinolytic bacteria from the rumen ecosystem found 109 colonies that produced clear zone, 84 colonies (86%) anaerobic and 17 colonies (14%) aerobic. Clear zone appeared in the third and fourth days inc...

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Main Authors: Sri Rahayu, FM Suhartati, EA Rimbawanto, Ning Iriyanti
Format: Article
Language:English
Published: Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science 2003-05-01
Series:Animal Production
Online Access:http://animalproduction.net/index.php/JAP/article/view/66
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spelling doaj-9f9959f6b1fc4d428dfdd46ac8291f6d2020-11-24T22:41:23Zeng Universitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production2541-58752541-58752003-05-015252Isolation and Identification of the Chitinolytic Bacteria from Rumen EcosystemSri RahayuFM SuhartatiEA RimbawantoNing IriyantiRumen is an interesting ecosystem for microbial exploration and their products. Isolation of the chitinolytic bacteria from the rumen ecosystem found 109 colonies that produced clear zone, 84 colonies (86%) anaerobic and 17 colonies (14%) aerobic. Clear zone appeared in the third and fourth days incubation. Four potential isolates were chosen for identification purposes. Results showed that the bacteria were sticky, gram-positive, motile, endospore-forming, mesophilic and aerobic. It was supposed to Bacillus spp. the optimal pH and temperature to produce chitinase from isolate 18 are pH 6.0 and temperature of 35-40ºC. Divalent cations Mg, Ca, Zn, and Mn increase chitinase activity, while Cu and Co inhibit enzyme activity. When isolate 18 was grown on shrimp waste meal, it showed aptimal activity on the fifth days incubation. (Animal Production 5(2): 73-78 (2003)   Key Words : Isolation, Identification, Chitinolytic Bacteria, Rumenhttp://animalproduction.net/index.php/JAP/article/view/66
collection DOAJ
language English
format Article
sources DOAJ
author Sri Rahayu
FM Suhartati
EA Rimbawanto
Ning Iriyanti
spellingShingle Sri Rahayu
FM Suhartati
EA Rimbawanto
Ning Iriyanti
Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem
Animal Production
author_facet Sri Rahayu
FM Suhartati
EA Rimbawanto
Ning Iriyanti
author_sort Sri Rahayu
title Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem
title_short Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem
title_full Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem
title_fullStr Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem
title_full_unstemmed Isolation and Identification of the Chitinolytic Bacteria from Rumen Ecosystem
title_sort isolation and identification of the chitinolytic bacteria from rumen ecosystem
publisher Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science
series Animal Production
issn 2541-5875
2541-5875
publishDate 2003-05-01
description Rumen is an interesting ecosystem for microbial exploration and their products. Isolation of the chitinolytic bacteria from the rumen ecosystem found 109 colonies that produced clear zone, 84 colonies (86%) anaerobic and 17 colonies (14%) aerobic. Clear zone appeared in the third and fourth days incubation. Four potential isolates were chosen for identification purposes. Results showed that the bacteria were sticky, gram-positive, motile, endospore-forming, mesophilic and aerobic. It was supposed to Bacillus spp. the optimal pH and temperature to produce chitinase from isolate 18 are pH 6.0 and temperature of 35-40ºC. Divalent cations Mg, Ca, Zn, and Mn increase chitinase activity, while Cu and Co inhibit enzyme activity. When isolate 18 was grown on shrimp waste meal, it showed aptimal activity on the fifth days incubation. (Animal Production 5(2): 73-78 (2003)   Key Words : Isolation, Identification, Chitinolytic Bacteria, Rumen
url http://animalproduction.net/index.php/JAP/article/view/66
work_keys_str_mv AT srirahayu isolationandidentificationofthechitinolyticbacteriafromrumenecosystem
AT fmsuhartati isolationandidentificationofthechitinolyticbacteriafromrumenecosystem
AT earimbawanto isolationandidentificationofthechitinolyticbacteriafromrumenecosystem
AT ningiriyanti isolationandidentificationofthechitinolyticbacteriafromrumenecosystem
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