Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study

Abstract Background Hormonal and enzymatic factors may render certain individuals more susceptible to orthodontically induced inflammatory root resorption (OIIRR). The objectives of this study are (1) to identify biochemical key markers in blood and saliva that may be correlated to the trend of exte...

Full description

Bibliographic Details
Main Authors: Dilara Yashin, Oyku Dalci, Mohammed Almuzian, Jenkin Chiu, Rajiv Ahuja, Apurv Goel, M. Ali. Darendeliler
Format: Article
Language:English
Published: SpringerOpen 2017-09-01
Series:Progress in Orthodontics
Online Access:http://link.springer.com/article/10.1186/s40510-017-0176-y
id doaj-9f9327929e2b4ea89f58eefd4beff0b7
record_format Article
spelling doaj-9f9327929e2b4ea89f58eefd4beff0b72020-11-24T21:08:44ZengSpringerOpenProgress in Orthodontics2196-10422017-09-011811810.1186/s40510-017-0176-yMarkers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-studyDilara Yashin0Oyku Dalci1Mohammed Almuzian2Jenkin Chiu3Rajiv Ahuja4Apurv Goel5M. Ali. Darendeliler6Discipline of Orthodontics, Faculty of Dentistry, University of SydneyDiscipline of Orthodontics, Faculty of Dentistry, University of SydneyDiscipline of Orthodontics, Faculty of Dentistry, University of SydneyDiscipline of Orthodontics, Faculty of Dentistry, University of SydneyDiscipline of Orthodontics, Faculty of Dentistry, University of SydneyAustralian Proteome Analysis Facility (APAF)Discipline of Orthodontics, Faculty of Dentistry, University of SydneyAbstract Background Hormonal and enzymatic factors may render certain individuals more susceptible to orthodontically induced inflammatory root resorption (OIIRR). The objectives of this study are (1) to identify biochemical key markers in blood and saliva that may be correlated to the trend of extensive OIIRR and (2) to utilise these markers to predict a susceptible patient-receiving orthodontic treatment. Methods Nine patients (mean age 23 + 2.9 years) who had moderate to severe OIIRR that assessed via orthopantomograms and met the inclusion criteria were classified as the root resorption group (RRG). Blood chemistry was evaluated using the collection of fasting blood and unstimulated saliva samples. Multiplex enzyme-linked immunosorbent assay (ELISA) arrays were used to screen blood and saliva samples for human cytokines, chemokines and several key enzymes that may play a role in root resorption following orthodontic force application. Biochemical findings from 16 matching subjects were used as the control (CG) for comparative measurements. Results Patients with moderate to severe OIIRR showed a significant increase in salivary cytokines including interleukin (IL) 7, IL-10, IL-12p70 and interferon-gamma (IFN-γ) level as well as a significant decrease in IL-4 level. Osteocalcin and procollagen type I N-terminal peptide (P1NP) appeared to be the only blood factors that showed a significant difference, more in the CG than the RRG. Conclusions Saliva might be a more valuable way of measuring changes in cytokine expression than blood secondary to orthodontic treatment. Although the increased expression of pro-inflammatory and anti-inflammatory cytokines may be determinants in the development of moderate to severe OIIRR, cytokine expression may be affected by several potential inflammations in another part of the body. Future research could investigate the cause/effect relationship of different cytokines, in a larger group of patients and at different time intervals, using digital subtraction radiography techniques and microfluidic biosensors.http://link.springer.com/article/10.1186/s40510-017-0176-y
collection DOAJ
language English
format Article
sources DOAJ
author Dilara Yashin
Oyku Dalci
Mohammed Almuzian
Jenkin Chiu
Rajiv Ahuja
Apurv Goel
M. Ali. Darendeliler
spellingShingle Dilara Yashin
Oyku Dalci
Mohammed Almuzian
Jenkin Chiu
Rajiv Ahuja
Apurv Goel
M. Ali. Darendeliler
Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
Progress in Orthodontics
author_facet Dilara Yashin
Oyku Dalci
Mohammed Almuzian
Jenkin Chiu
Rajiv Ahuja
Apurv Goel
M. Ali. Darendeliler
author_sort Dilara Yashin
title Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
title_short Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
title_full Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
title_fullStr Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
title_full_unstemmed Markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
title_sort markers in blood and saliva for prediction of orthodontically induced inflammatory root resorption: a retrospective case controlled-study
publisher SpringerOpen
series Progress in Orthodontics
issn 2196-1042
publishDate 2017-09-01
description Abstract Background Hormonal and enzymatic factors may render certain individuals more susceptible to orthodontically induced inflammatory root resorption (OIIRR). The objectives of this study are (1) to identify biochemical key markers in blood and saliva that may be correlated to the trend of extensive OIIRR and (2) to utilise these markers to predict a susceptible patient-receiving orthodontic treatment. Methods Nine patients (mean age 23 + 2.9 years) who had moderate to severe OIIRR that assessed via orthopantomograms and met the inclusion criteria were classified as the root resorption group (RRG). Blood chemistry was evaluated using the collection of fasting blood and unstimulated saliva samples. Multiplex enzyme-linked immunosorbent assay (ELISA) arrays were used to screen blood and saliva samples for human cytokines, chemokines and several key enzymes that may play a role in root resorption following orthodontic force application. Biochemical findings from 16 matching subjects were used as the control (CG) for comparative measurements. Results Patients with moderate to severe OIIRR showed a significant increase in salivary cytokines including interleukin (IL) 7, IL-10, IL-12p70 and interferon-gamma (IFN-γ) level as well as a significant decrease in IL-4 level. Osteocalcin and procollagen type I N-terminal peptide (P1NP) appeared to be the only blood factors that showed a significant difference, more in the CG than the RRG. Conclusions Saliva might be a more valuable way of measuring changes in cytokine expression than blood secondary to orthodontic treatment. Although the increased expression of pro-inflammatory and anti-inflammatory cytokines may be determinants in the development of moderate to severe OIIRR, cytokine expression may be affected by several potential inflammations in another part of the body. Future research could investigate the cause/effect relationship of different cytokines, in a larger group of patients and at different time intervals, using digital subtraction radiography techniques and microfluidic biosensors.
url http://link.springer.com/article/10.1186/s40510-017-0176-y
work_keys_str_mv AT dilarayashin markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
AT oykudalci markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
AT mohammedalmuzian markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
AT jenkinchiu markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
AT rajivahuja markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
AT apurvgoel markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
AT malidarendeliler markersinbloodandsalivaforpredictionoforthodonticallyinducedinflammatoryrootresorptionaretrospectivecasecontrolledstudy
_version_ 1716759618025160704