A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
<p>Abstract</p> <p>Background</p> <p>In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potential differences in the amplification performance of plasmid DNA com...
Main Authors: | Haugland Richard A, Varma Manju, Seifring Shawn, Sivaganesan Mano, Shanks Orin C |
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Format: | Article |
Language: | English |
Published: |
BMC
2008-02-01
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Series: | BMC Bioinformatics |
Online Access: | http://www.biomedcentral.com/1471-2105/9/120 |
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