A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards

<p>Abstract</p> <p>Background</p> <p>In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potential differences in the amplification performance of plasmid DNA com...

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Bibliographic Details
Main Authors: Haugland Richard A, Varma Manju, Seifring Shawn, Sivaganesan Mano, Shanks Orin C
Format: Article
Language:English
Published: BMC 2008-02-01
Series:BMC Bioinformatics
Online Access:http://www.biomedcentral.com/1471-2105/9/120

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