A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression
Abstract Background Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially available eukaryotic in vitro translation systems by its flexibility, hig...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2020-03-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12896-020-00610-5 |