A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression

Abstract Background Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially available eukaryotic in vitro translation systems by its flexibility, hig...

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Bibliographic Details
Main Authors: Szilvia Krisztina Nagy, Brigitta Margit Kállai, Judit András, Tamás Mészáros
Format: Article
Language:English
Published: BMC 2020-03-01
Series:BMC Biotechnology
Subjects:
TEV
Online Access:http://link.springer.com/article/10.1186/s12896-020-00610-5