Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides
A total 146 isolates of bacteria were taken from leaf surface, fruit skin, and blossom of mango (var. Nam Dorkmai). They were tested for the inhibition of mycelial growth of Colletotrichum gloeosporioides, a causal agent of anthracnose, on potato dextrose agar (PDA). Seventy-four bacterial isolates...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Walailak University
2011-12-01
|
Series: | Walailak Journal of Science and Technology |
Subjects: | |
Online Access: | http://wjst.wu.ac.th/index.php/wjst/article/view/186 |
id |
doaj-9f79108e63b44ebf9d56b89514c65b49 |
---|---|
record_format |
Article |
spelling |
doaj-9f79108e63b44ebf9d56b89514c65b492020-11-25T02:17:48ZengWalailak UniversityWalailak Journal of Science and Technology1686-39332228-835X2011-12-011210.2004/wjst.v1i2.186179Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioidesPrakong YENJIT0Wanwilai INTANOO1Chiradej CHAMSWARNG2Jingtair SIRIPANICH3Warin INTANA4Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140Department of Horticulture, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161A total 146 isolates of bacteria were taken from leaf surface, fruit skin, and blossom of mango (var. Nam Dorkmai). They were tested for the inhibition of mycelial growth of Colletotrichum gloeosporioides, a causal agent of anthracnose, on potato dextrose agar (PDA). Seventy-four bacterial isolates inhibited the growth of fungal mycelia by 24.51-49.10%. The 40 highly effective isolates out of 74 isolates were further tested for the potential to reduce the development of anthracnose lesion on detached leaves of mango marcotages at 24 h after inoculation of pathogen. Results indicated that 12 isolates provided high efficacy for inhibiting disease by 51.39-86.11%. Application of these bacteria on mango fruits at 24 h prior to the inoculation of the pathogen revealed that isolates B46 and B12 suppressed disease by 50.36 and 44.13% respectively while Trichoderma harzianum CB-Pin-01 provided 37.30% of the inhibition. For controlling post-harvest disease, an isolate B12 or B12 integrated with hot water treatment (55 oC) provided 91.33 and 88.00% of disease severity reduction respectively when applied at 24 h before inoculation of pathogen. Isolates B12 and B44 were identified as Bacillus subtilis while B46 and K112 were B. licheniformis and B. cereus respectively. The mechanism of these isolates for controlling C. gloeosporioides was the reduction of spore germination and the inhibition of germ-tube elongation.http://wjst.wu.ac.th/index.php/wjst/article/view/186AntagonistColletotrichum gloeosporioidesMango diseaseDisease suppressionBiocontrol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Prakong YENJIT Wanwilai INTANOO Chiradej CHAMSWARNG Jingtair SIRIPANICH Warin INTANA |
spellingShingle |
Prakong YENJIT Wanwilai INTANOO Chiradej CHAMSWARNG Jingtair SIRIPANICH Warin INTANA Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides Walailak Journal of Science and Technology Antagonist Colletotrichum gloeosporioidesMango disease Disease suppression Biocontrol |
author_facet |
Prakong YENJIT Wanwilai INTANOO Chiradej CHAMSWARNG Jingtair SIRIPANICH Warin INTANA |
author_sort |
Prakong YENJIT |
title |
Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides |
title_short |
Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides |
title_full |
Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides |
title_fullStr |
Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides |
title_full_unstemmed |
Use of Promising Bacterial Strains for Controlling Anthracnose on Leaf and Fruit of Mango Caused by Colletotrichum gloeosporioides |
title_sort |
use of promising bacterial strains for controlling anthracnose on leaf and fruit of mango caused by colletotrichum gloeosporioides |
publisher |
Walailak University |
series |
Walailak Journal of Science and Technology |
issn |
1686-3933 2228-835X |
publishDate |
2011-12-01 |
description |
A total 146 isolates of bacteria were taken from leaf surface, fruit skin, and blossom of mango (var. Nam Dorkmai). They were tested for the inhibition of mycelial growth of Colletotrichum gloeosporioides, a causal agent of anthracnose, on potato dextrose agar (PDA). Seventy-four bacterial isolates inhibited the growth of fungal mycelia by 24.51-49.10%. The 40 highly effective isolates out of 74 isolates were further tested for the potential to reduce the development of anthracnose lesion on detached leaves of mango marcotages at 24 h after inoculation of pathogen. Results indicated that 12 isolates provided high efficacy for inhibiting disease by 51.39-86.11%. Application of these bacteria on mango fruits at 24 h prior to the inoculation of the pathogen revealed that isolates B46 and B12 suppressed disease by 50.36 and 44.13% respectively while Trichoderma harzianum CB-Pin-01 provided 37.30% of the inhibition. For controlling post-harvest disease, an isolate B12 or B12 integrated with hot water treatment (55 oC) provided 91.33 and 88.00% of disease severity reduction respectively when applied at 24 h before inoculation of pathogen. Isolates B12 and B44 were identified as Bacillus subtilis while B46 and K112 were B. licheniformis and B. cereus respectively. The mechanism of these isolates for controlling C. gloeosporioides was the reduction of spore germination and the inhibition of germ-tube elongation. |
topic |
Antagonist Colletotrichum gloeosporioidesMango disease Disease suppression Biocontrol |
url |
http://wjst.wu.ac.th/index.php/wjst/article/view/186 |
work_keys_str_mv |
AT prakongyenjit useofpromisingbacterialstrainsforcontrollinganthracnoseonleafandfruitofmangocausedbycolletotrichumgloeosporioides AT wanwilaiintanoo useofpromisingbacterialstrainsforcontrollinganthracnoseonleafandfruitofmangocausedbycolletotrichumgloeosporioides AT chiradejchamswarng useofpromisingbacterialstrainsforcontrollinganthracnoseonleafandfruitofmangocausedbycolletotrichumgloeosporioides AT jingtairsiripanich useofpromisingbacterialstrainsforcontrollinganthracnoseonleafandfruitofmangocausedbycolletotrichumgloeosporioides AT warinintana useofpromisingbacterialstrainsforcontrollinganthracnoseonleafandfruitofmangocausedbycolletotrichumgloeosporioides |
_version_ |
1724884980949057536 |