| Summary: | Innate immune molecules, SP-A1 (6A<sup>2</sup>, 6A<sup>4</sup>) and SP-A2 (1A<sup>0</sup>, 1A<sup>3</sup>), differentially affect young mouse survival after infection. Here, we investigated the impact of SP-A variants on the survival of aged mice. hTG mice carried a different SP-A1 or SP-A2 variant and SP-A-KO were either infected with <i>Klebsiella pneumoniae</i> or exposed to filtered air (FA) or ozone (O<sub>3</sub>) prior to infection, and their survival monitored over 14 days. In response to infection alone, no gene- or sex-specific (except for 6A<sup>2</sup>) differences were observed; variant-specific survival was observed (1A<sup>0</sup> > 6A<sup>4</sup>). In response to O<sub>3</sub>, gene-, sex-, and variant-specific survival was observed with SP-A2 variants showing better survival in males than females, and 1A<sup>0</sup> females > 1A<sup>3</sup> females. A serendipitous, and perhaps clinically important observation was made; mice exposed to FA prior to infection exhibited significantly better survival than infected alone mice. 1A<sup>0</sup> provided an overall better survival in males and/or females indicating a differential role for SP-A genetics. Improved ventilation, as provided by FA, resulted in a survival of significant magnitude in aged mice and perhaps to a lesser extent in young mice. This may have clinical application especially within the context of the current pandemic.
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