A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2
We optimized and tested a postbioprocessing step with a single-culture archaeon to upgrade biogas (i.e., increase methane content) from anaerobic digesters via conversion of CO2 into CH4 by feeding H2 gas. We optimized a culture of the thermophilic methanogen Methanothermobacter thermautotrophicus u...
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Online Access: | http://dx.doi.org/10.1155/2013/157529 |
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doaj-9f19db1950a14e859a569b68cc4bd6f32021-07-02T02:54:34ZengHindawi LimitedArchaea1472-36461472-36542013-01-01201310.1155/2013/157529157529A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2Matthew R. Martin0Jeffrey J. Fornero1Rebecca Stark2Laurens Mets3Largus T. Angenent4Electrochaea, LLC, 1005 N. Warson Road, Suite 201, St. Louis, MO 63132, USAElectrochaea, LLC, 1005 N. Warson Road, Suite 201, St. Louis, MO 63132, USAElectrochaea, LLC, 1005 N. Warson Road, Suite 201, St. Louis, MO 63132, USAThe University of Chicago, 920 E. 58th Street, Chicago, IL 60637, USACornell University, 214 Riley-Robb Hall, Ithaca, NY 14853, USAWe optimized and tested a postbioprocessing step with a single-culture archaeon to upgrade biogas (i.e., increase methane content) from anaerobic digesters via conversion of CO2 into CH4 by feeding H2 gas. We optimized a culture of the thermophilic methanogen Methanothermobacter thermautotrophicus using: (1) a synthetic H2/CO2 mixture; (2) the same mixture with pressurization; (3) a synthetic biogas with different CH4 contents and H2; and (4) an industrial, untreated biogas and H2. A laboratory culture with a robust growth (dry weight of 6.4–7.4 g/L; OD600 of 13.6–15.4), a volumetric methane production rate of 21 L/L culture-day, and a H2 conversion efficiency of 89% was moved to an industrial anaerobic digester facility, where it was restarted and fed untreated biogas with a methane content of ~70% at a rate such that CO2 was in excess of the stoichiometric requirements in relation to H2. Over an 8-day operating period, the dry weight of the culture initially decreased slightly before stabilizing at an elevated level of ~8 g/L to achieve a volumetric methane production rate of 21 L/L culture-day and a H2 conversion efficiency of 62%. While some microbial contamination of the culture was observed via microscopy, it did not affect the methane production rate of the culture.http://dx.doi.org/10.1155/2013/157529 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthew R. Martin Jeffrey J. Fornero Rebecca Stark Laurens Mets Largus T. Angenent |
spellingShingle |
Matthew R. Martin Jeffrey J. Fornero Rebecca Stark Laurens Mets Largus T. Angenent A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2 Archaea |
author_facet |
Matthew R. Martin Jeffrey J. Fornero Rebecca Stark Laurens Mets Largus T. Angenent |
author_sort |
Matthew R. Martin |
title |
A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2 |
title_short |
A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2 |
title_full |
A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2 |
title_fullStr |
A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2 |
title_full_unstemmed |
A Single-Culture Bioprocess of Methanothermobacter thermautotrophicus to Upgrade Digester Biogas by CO2-to-CH4 Conversion with H2 |
title_sort |
single-culture bioprocess of methanothermobacter thermautotrophicus to upgrade digester biogas by co2-to-ch4 conversion with h2 |
publisher |
Hindawi Limited |
series |
Archaea |
issn |
1472-3646 1472-3654 |
publishDate |
2013-01-01 |
description |
We optimized and tested a postbioprocessing step with a single-culture archaeon to upgrade biogas (i.e., increase methane content) from anaerobic digesters via conversion of CO2 into CH4 by feeding H2 gas. We optimized a culture of the thermophilic methanogen Methanothermobacter thermautotrophicus using: (1) a synthetic H2/CO2 mixture; (2) the same mixture with pressurization; (3) a synthetic biogas with different CH4 contents and H2; and (4) an industrial, untreated biogas and H2. A laboratory culture with a robust growth (dry weight of 6.4–7.4 g/L; OD600 of 13.6–15.4), a volumetric methane production rate of 21 L/L culture-day, and a H2 conversion efficiency of 89% was moved to an industrial anaerobic digester facility, where it was restarted and fed untreated biogas with a methane content of ~70% at a rate such that CO2 was in excess of the stoichiometric requirements in relation to H2. Over an 8-day operating period, the dry weight of the culture initially decreased slightly before stabilizing at an elevated level of ~8 g/L to achieve a volumetric methane production rate of 21 L/L culture-day and a H2 conversion efficiency of 62%. While some microbial contamination of the culture was observed via microscopy, it did not affect the methane production rate of the culture. |
url |
http://dx.doi.org/10.1155/2013/157529 |
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